Medical Laboratory Journal
Medical Laboratory Journal
mljgoums
Medical Sciences
http://mlj.goums.ac.ir
1
admin
2538-4449
10.52547/mlj
en
jalali
1394
12
1
gregorian
2016
3
1
10
2
online
1
fulltext
fa
Analysis of Plasma Amino Acids Using RP-HPLC and Pre-Column Derivatization with OPA/3-MPA
تحقيقي
Original Paper
<div style="margin-left: -7.1pt">
<p><strong>ABSTRACT</strong></p>
<p><strong> Background and Objective:</strong> Measurement of amino acids is an important tool for metabolic studies and evaluation of patients’ clinical condition. The aim of this study was to analyze the plasma amino acids using reverse phase high performance liquid chromatography techniques (RP-HPLC) with pre-column derivatization by o-phthalaldehyde (OPA) in combination with 3- mercaptopropionic acid (3-MPA).</p>
<p><strong> Methods:</strong> Overall, 107 neonates and babies suspected of having metabolic disorder were enrolled in this study. The level of amino acids in plasma samples was analyzed within 65 minutes by HPLC with pre-column derivatization by OPA/3-MPA. This was a gradient RP-HPLC method that was performed using two solvents with a ratio of methanol and sodium acetate. L-norvaline internal standard was used as the reference peak for amino acids. Standard mixture of amino acids was used to determine the concentrations of amino acids.</p>
<p><strong> Results:</strong> According to the values of coefficient of variation obtained for each amino acid, the results indicated a good chromatographic separation of amino acids by this method. The use of OPA/3-MPA derivative reagent increased the efficiency and resolution of amino acids chromatographic separation.</p>
<p><strong> Conclusion:</strong> Due to simple preparation and accurate assessment, determination of plasma amino acids using OPA/3-MPA derivatives and RP-HPLC is a suitable method in many clinical samples.</p>
<p></p>
</div>
High Pressure Liquid Chromatography, OPA/3MPA, Amino Acids.
52
57
http://mlj.goums.ac.ir/browse.php?a_code=A-10-1-394&slc_lang=fa&sid=1
Fahime
Mohammad Abadi
100319475328460011610
100319475328460011610
No
Biochemistry & Metabolic Disorders Research Center, Golestan University of Medical Sciences, Gorgan, Iran
Arezoo
Mirfazeli
100319475328460011611
100319475328460011611
No
Cognitional Malformations Research Center, Golestan University of Medical Sciences, Gorgan, Iran
Hossein
Zaeri
100319475328460011612
100319475328460011612
No
Cognitional Malformations Research Center, Golestan University of Medical Sciences, Gorgan, Iran
Mojgan
Nejabat
100319475328460011613
100319475328460011613
No
School of pharmacy, Mashhad university of Midical Science, Mashhad, Iran
Mahsa
taherizadeh
100319475328460011614
100319475328460011614
No
Biochemistry & Metabolic Disorders Research Center, Golestan University of Medical Sciences, Gorgan, Iran
Mohammad
Ariaie
100319475328460011615
100319475328460011615
No
Golestan University of Medical Sciences, Gorgan, Iran
azadeh
Aliarab
100319475328460011616
100319475328460011616
No
Hamidreza
Joshaghani
joshaghani@goums.ac.ir
100319475328460011617
100319475328460011617
Yes
Laboratory Sciences Research Center, Golestan University of Medical Science, Gorgan, Iran