Medical Laboratory Journal
Medical Laboratory Journal
mljgoums
Medical Sciences
http://mlj.goums.ac.ir
1
admin
2538-4449
10.52547/mlj
en
jalali
1393
10
1
gregorian
2015
1
1
8
5
online
1
fulltext
fa
کلونینگ ژن اتولیزین ماینور استرپتوکوکوس پنومونیه
Cloning of Minor Autolysin of Streptococcus Pneumoniae
تحقيقي
Original Paper
Abstract Background and Objective: Increased antibiotic resistant strains and inadequacy of current vaccines against pneumococcal infections necessitate the study of novel protein antigens. It seems that minor autolysin of Streptococcus pneumoniae may have antigenicity. Thus, we aimed at cloning its gene for the first time. Material and Methods: After DNA extraction of Streptococcus pneumoniae (ATCC 49619), Specific primers were designed for amplifying minor autolysin gene fragment, using PCR. The purified gene fragment was inserted into pET21a vector and was transformed into bacterial competent cells by heat shock technique. The presence of gene and absence of mutation in the recombinant vector were checked out with sequencing and enzymatic digestion methods. The gene sequence was finally analyzed by bioinformatic tools. Results: The gene of minor autolysin was cloned successfully and the result of enzymatic digestion was the indication of complete isolation of this gen from plasmid. . Bioinformatics studies revealed that the mature protein was lacking signal peptide and the gene encoded 318 amino acids with a molecular weight of 36.4 kDa. Conclusion: The presentation and characterization of novel antigens such as minor autolysin could help us with finding new approaches for preventing and controlling pneumococcal infection. Keywords: Streptococcus Pneumoniae, Minor Autolysin, Cloning
Streptococcus Pneumoniae, Minor Autolysin, Cloning
104
110
http://mlj.goums.ac.ir/browse.php?a_code=A-10-1-279&slc_lang=fa&sid=1
Mahboobi, R
رامینا محبوبی
100319475328460012684
100319475328460012684
No
Tehran University of Medical Sciences, Tehran, Iran
دانشگاه علوم پزشکی تهران
Fallah Mehrabadi, J
جلیل فلاح مهرآبادی
100319475328460012685
100319475328460012685
No
Lister Microbiology Institute, Tehran, Iran
موسسه میکروب شناسی لیستر،تهران
نوشین قنبری
100319475328460012686
100319475328460012686
No
علوم پزشکی کرمانشاه
Pourmand, MR
محمدرضا پورمند
mpourmand@tums.ac.ir
100319475328460012687
100319475328460012687
Yes
School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
دانشگاه علوم پزشکی تهران
Mashhadi, R
راحیل مشهدی
100319475328460012688
100319475328460012688
No
Tehran University of Medical Sciences, Tehran, Iran
دانشگاه علوم پزشکی تهران
Haddadi, A
آذر حدادی
100319475328460012689
100319475328460012689
No
Sina Hospital, Tehran University of Medical Sciences, Tehran, Iran
دانشگاه علوم پزشکی تهران