en بیوشیمی Biochemistry Research Article Research Article <span style="font-size:14px;"><span style="font-family:Times New Roman;"><b>Background:</b> <span style="color:black">Research has shown that the extract of some plants has an effective protective effect on liver cells against toxins and oxidants. In this research, the effect of hydroalcoholic extract of ginger against hepatotoxicity caused by doxorubicin (Dox) in adult male rats was investigated.</span><br> <b>Methods:</b> In this experimental study, 63 adult male rats were divided into 7 groups (n = 9 per group). The experimental treatments included the control and placebo groups without the use of medicines, experimental group I that received Dox at a dose of 20 mg/kg, experimental groups II and III with 300 and 600 mg/kg of ginger hydroalcoholic extract, and experimental groups IV and V that received 20 mg/kg Dox + 300 and 600 mg/kg of ginger hydroalcoholic extract, respectively. After the end of the test period, serum levels of total bilirubin and direct bilirubin were measured. In addition, the histological changes in the liver were examined after hematoxylin and eosin (H&E) staining.<br> <b>Results:</b> Serum levels of total bilirubin and direct bilirubin in the Dox group showed a significant increase compared to the control group. In contrast, serum levels of total bilirubin and direct bilirubin in Dox + ZIN 600 and Dox + ZIN 300&nbsp; had a significant reduction compared to the Dox group (P < 0.05). Ginger extract prevented apoptosis and Dox-induced liver tissue damage in dose-dependent designs.<br> <b>Conclusion</b>: The hydroalcoholic extract of ginger improves the changes of serum bilirubin and liver tissue after receiving Dox due to its antioxidant compounds.</span></span> Ginger Extract, Doxorubicin, Adverse effects, Liver failure, Rat 9 12 http://mlj.goums.ac.ir/browse.php?a_code=A-10-1299-1&slc_lang=en&sid=1 Marzieh Niakan mrs.marziehniakan@gmail.com 100319475328460031903 100319475328460031903 No Department of Biology, Kazerun Branch, Islamic Azad University, Kazerun, Iran Habib Alla Johari hjowhary@yahoo.co.uk 100319475328460031904 100319475328460031904 Yes Department of Biology, Darab Branch, Islamic Azad University, Darab, Iran Mehrdad Shariati mehrdadshariati@kau.ac.ir 100319475328460031905 100319475328460031905 No Department of Biology, Kazerun Branch, Islamic Azad University, Kazerun, Iran Davood Moghadamnia Dmansouri@yahoo.com 100319475328460031906 100319475328460031906 No Department of Biology, Shiraz Branch, Islamic Azad University, Shiraz,Iran Ebrahim Talebi Ebrahim.Talebi@iau.ac.ir 100319475328460031907 100319475328460031907 No Department of Animal Sciences, Darab Branch, Islamic Azad University, Darab, Fars, Iran