en بیوشیمی Biochemistry Research Article Research Article <div style="text-align: justify;"><span style="font-size:12px;"><span style="font-family:Times New Roman;"><span style="line-height:normal"><span calibri=""><b><span style="font-family:"Times New Roman","serif""></span></b></span></span><span style="line-height:200%"><span calibri=""><b><span style="line-height:200%"><span new="" roman="" times="">Background</span></span></b><span style="line-height:200%"><span new="" roman="" times="">: Genetic studies have indicated the effective role of transcription factors in insulin synthesis and secretion, especially in the case of diabetes. This study aimed to assess the effects of high-intensity interval training on transcription factor 7-like 2/ glucagon-like peptide 1 (TCF7L2 / GLP-1) axis in pancreatic tissue of obese rats with type 2 diabetes mellitus (T2DM).</span></span><span style="line-height:200%"><span style="font-family:"Times New Roman",serif"></span></span></span></span><br> <span style="line-height:200%"><span calibri=""><b><span style="line-height:200%"><span new="" roman="" times="">Methods: </span></span></b><span style="line-height:200%"><span new="" roman="" times="">For this purpose, obesity was induced in 21 male Wistar rats (weighting 220&plusmn;10 g) by exposure to a high-fat diet for six weeks. Then, the rats were randomly assigned to a non-diabetic, a control T2DM, and an exercise diabetic group. Next, T2DM was induced by </span></span><em><span style="line-height:200%"><span style="font-style:normal">intraperitoneal injection of streptozotocin</span></span></em><span style="line-height:200%"><span new="" roman="" times=""> (25 mg/kg). The rats in the exercise group participated in a HIIT program, five times a week, for six weeks. After the intervention, TCF7L2 and GLP1 expression</span></span> <span style="line-height:200%"><span new="" roman="" times="">in the pancreas tissue was determined by real-time PCR. Serum insulin, glucose, and beta cell function were compared between the study groups. Data were analyzed using one-way ANOVA and Tukey post hoc test at a significance level of 0.05.</span></span><span style="line-height:200%"><span style="font-family:"Times New Roman",serif"></span></span></span></span><br> <span style="line-height:200%"><span calibri=""><b><span style="line-height:200%"><span new="" roman="" times="">Results: </span></span></b><span style="line-height:200%"><span new="" roman="" times="">Induction of T2DM increased glucose level and TCF7L2 expression but decreased insulin, beta cell function, and GLP-1R expression. In addition, HIIT significantly decreased TCF7L2 expression as well as glucose level, serum insulin, and beta cell function; however, it did not significantly change GLP-1R expression compared with the control diabetes rats.</span></span></span></span><br> <span style="line-height:200%"><span calibri=""><b><span style="line-height:200%"><span new="" roman="" times="">Conclusion: </span></span></b><span style="line-height:200%"><span new="" roman="" times="">Based on the findings, the improvement of serum insulin and glucose level following HIIT may be attributed to the decrease in TCF7L2 gene expression in the pancreatic tissue of diabetic rats.</span></span></span></span></span></span></div> Insulin,Exercise,Gene expression, 15 21 http://mlj.goums.ac.ir/browse.php?a_code=A-10-956-2&slc_lang=en&sid=1 Mojtaba Eizadi izadimojtaba2006@yahoo.com 100319475328460031996 100319475328460031996 Yes Department of Exercise Physiology, Saveh Branch, Islamic Azad University, Saveh, Iran Mehdy Behkar mehdibehkar@yahoo.com 100319475328460031997 100319475328460031997 No Department of Exercise Physiology, Islamshahr Branch, Islamic Azad University, Tehran, Iran Yaser Kazemzadeh yaser.kazemzadeh@yahoo.com 100319475328460031998 100319475328460031998 No Department of Exercise Physiology, Islamshahr Branch, Islamic Azad University, Tehran, Iran Saeid sedaghaty sa49sd@gmail.com 100319475328460031999 100319475328460031999 No Department of Exercise Physiology, Islamshahr Branch, Islamic Azad University, Tehran, Iran Motahareh Moslehi phd.moslehi@gmail.com 100319475328460032000 100319475328460032000 No Department of Exercise Physiology, Islamshahr Branch, Islamic Azad University, Tehran, Iran