en بیوشیمی Biochemistry Research Article Research Article <span style="font-size:11pt"><span style="line-height:200%"><span calibri="" style="font-family:"><b><span style="font-size:12.0pt"><span style="line-height:200%"><span new="" roman="" style="font-family:" times="">Background and objectives:</span></span></span></b><span style="font-size:12.0pt"><span style="line-height:200%"><span new="" roman="" style="font-family:" times=""> Non-alcoholic fatty liver disease (NAFLD) is a chronic liver disease caused by the accumulation of large amounts of fat in the hepatocytes. Given that atorvastatin is effective for treatment of NAFLD, the present study investigated effects of high-fat/fructose diet (HFFD) with atorvastatin on liver enzymes and lipid profile in a NAFLD rat model.</span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:200%"><span calibri="" style="font-family:"><b><span style="font-size:12.0pt"><span style="line-height:200%"><span new="" roman="" style="font-family:" times="">Methods: </span></span></span></b><span style="font-size:12.0pt"><span style="line-height:200%"><span new="" roman="" style="font-family:" times="">Thirty-two male Wistar rats were divided into four groups: 1) normal control, 2) HFFD control, 3) HFFD + atorvastatin, and 4) normal + atorvastatin. The groups received HFFD for 15 weeks to induce hepatosteatosis. Atorvastatin was administrated at the dose of 10 mg/kg/day. Lipid profile and liver enzymes were measured after eight weeks of intervention.</span></span></span><span style="font-size:12.0pt"><span style="line-height:200%"><span style="font-family:"Times New Roman","serif""></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:200%"><span calibri="" style="font-family:"><b><span style="font-size:12.0pt"><span style="line-height:200%"><span new="" roman="" style="font-family:" times="">Results: </span></span></span></b><span style="font-size:12.0pt"><span style="line-height:200%"><span new="" roman="" style="font-family:" times="">Triglyceride, cholesterol, gamma-glutamyl transferase, and aspartate transaminase were significantly reduced in the HFFD + atorvastatin group compared with the HFFD control group. In addition, cholesterol, high-density lipoprotein, alkaline phosphatase, and gamma-glutamyl transferase were significantly increased in the normal + atorvastatin group compared with the normal control group. Low-density lipoprotein increased significantly in the HFFD + atorvastatin group and the normal + atorvastatin group compared with other groups. There was a significant difference in the alanine transaminase levels between the groups taking atorvastatin. In fact, alanine transaminase level was lowest in the normal + atorvastatin group.</span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:200%"><span calibri="" style="font-family:"><b><span style="font-size:12.0pt"><span style="line-height:200%"><span new="" roman="" style="font-family:" times="">Conclusion: </span></span></span></b><span style="font-size:12.0pt"><span style="line-height:200%"><span new="" roman="" style="font-family:" times="">Atorvastatin improves the lipid profile and fatty liver and controls liver enzymes. Therefore, it can be used with caution to improve the lipid profile and reduce the complications of NAFLD.</span></span></span></span></span></span> Atorvastatin,Diet, High-Fat,Non-alcoholic Fatty Liver Disease, 7 13 http://mlj.goums.ac.ir/browse.php?a_code=A-10-1081-1&slc_lang=en&sid=1 Zahra Eslami z.eslami@goums.ac.ir 100319475328460022283 100319475328460022283 No Metabolic Disorders Research Center, Golestan University of Medical Sciences, Gorgan, Golestan province, Iran Yahya Mohammadnajad Panah kandi yahya_mnp@yahoo.com 100319475328460022284 100319475328460022284 No Department of Sport PhysiologyIslamic Azad University, Islamshahr Branch, Islamshahr, Iran Alireza Norouzi norozi@goums.ac.ir 100319475328460022285 100319475328460022285 No Golestan Research Center of Gastroenterology and Hepatology, GolestanUniversity of Medical Sciences, Gorgan, Iran Abdorreza Eghbal Moghanlou reza.eghbal61@yahoo.com 100319475328460022286 100319475328460022286 No farhangian University, Ardebil, Iran Mehdi Sheikh arabi msheykharabi@yahoo.com 100319475328460022287 100319475328460022287 No Golestan University of Medical Sciences and Health Services, Gorgan, Iran Vahideh Kazeminejad vahidehkazeminejad@yahoo.com 100319475328460022288 100319475328460022288 No Department of phatology, Golestan University of Medical Sciences and Health Services, Gorgan, Iran seyedeh somayeh hosseini alarzi somaye2631@yahoo.com 100319475328460022289 100319475328460022289 No Laboratory Science research center, faculty of Paramedicine of Golestan University of medical sciences, Gorgan, Iran Aref Saeidi 100319475328460022290 100319475328460022290 No Department of Sport Physiotraphy, University of Lahore, Panjab, Pakistan Hamidreza Joshaghani hr.joshaghani99@gmail.com 100319475328460022291 100319475328460022291 Yes Laboratory Science research center, faculty of Paramedicine of Golestan University of medical sciences, Gorgan, Iran