Medical Laboratory Journal
Medical Laboratory Journal
mljgoums
Medical Sciences
http://mlj.goums.ac.ir
1
admin
2538-4449
10.52547/mlj
en
jalali
1400
10
1
gregorian
2022
1
1
16
1
online
1
fulltext
en
Effects of Alginate Coating and Neutral Electrolyzed Water on E. coli O157:H7 Contamination of Salmon Fillets
ميکروب شناسي
Microbiology
Research Article
Research Article
<strong>Background and objectives:</strong> Neutralized electrolyzed water (NEW) is a novel natural disinfectant. It has been suggested that application of NEW can improve the shelf life of fish. This study aimed to investigate effect of NEW incorporated in alginate coating on growth of <em>Escherichia coli </em>O<sub>157</sub>: H<sub>7</sub> on salmon fillets over a period of 12 days.<br>
<strong>Methods:</strong> Fish fillets were inoculated with <em>E. coli </em>O<sub>157</sub>:H<sub>7</sub> and divided into six different treatment groups: control (no coating), distilled water, alginate, EW, EW & alginate (Samples coated with alginate solution prepared by EW), and EW+ alginate (samples immersed in EW, then coated with alginate solution). The fillets were kept at 4 °C, and the bacterial count was determined on days: 0, 2, 4, 8, and 12. Data analysis was performed using repeated ANOVA and Bonferroni post-hoctest at statistical significance of 0.05.<br>
<strong>Results:</strong> Treatment with alginate coating and EW alone could significantly reduce <em>E. coli </em>O<sub>157</sub>: H<sub>7</sub> count on the salmon fillets. However, maximum reduction (1.27 log CFU/g) of bacteria was achieved when using alginate coating combined with EW.<br>
<strong>Conclusion:</strong> According to the results, the combination of alginate coating with EW can be applied as a natural antimicrobial for increasing safety of food products, especially fish, against pathogenic bacteria such as <em>E. coli</em> O157: H7.
Alginates,Escherichia coli O157,Salmon,
20
24
http://mlj.goums.ac.ir/browse.php?a_code=A-10-424-2&slc_lang=en&sid=1
romina
Saei Hamedani
romiina.1992.1992@gmail.com
100319475328460021458
100319475328460021458
No
Department of Food Hygiene and Aquaculture, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
saeid
khanzadi
khanzadi@um.ac.ir
100319475328460021459
100319475328460021459
Yes
Department of Food Hygiene and Aquaculture, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
mohammad
hashemi
hashemiMD@mums.ac.ir
100319475328460021460
100319475328460021460
No
Department of Nutrition, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
mohammad
azizzadeh
Azizzadeh@gmail.com
100319475328460021461
100319475328460021461
No
Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Iran