en ميکروب شناسي Microbiology Research Article Research Article <div style="text-align: justify;"><span style="font-size:12px;"><span style="font-family:Times New Roman;"><span style="line-height:200%"><span style="unicode-bidi:embed"><span calibri=""><b><span style="background:white"><span style="line-height:200%"><span new="" roman="" times=""><span style="color:black">Background:</span></span></span></span></b><span style="background:white"><span style="line-height:200%"><span new="" roman="" times=""><span style="color:black"> Overuse and misuse of antibiotics in the agricultural and healthcare sectors have led to the emergence of antibiotic-resistant strains. Therefore, finding alternative antimicrobial compounds, such as phytochemicals, is of great importance. This study evaluated the </span></span></span></span><span style="line-height:200%"><span new="" roman="" times="">feasibility of carvacrol as an antifungal agent in suppressing the </span></span><span style="background:white"><span style="line-height:200%"><span new="" roman="" times=""><span style="color:black">planktonic and hyphal </span></span></span></span><span style="line-height:200%"><span new="" roman="" times="">growth of clinical isolates of fluconazole<span style="background:white"><span style="color:black">-susceptible and -resistant </span></span><i>Candida tropicalis</i>. </span></span><span style="line-height:200%"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span><br> <span style="line-height:200%"><span style="unicode-bidi:embed"><span calibri=""><b><span style="line-height:200%"><span new="" roman="" times="">Methods:</span></span></b><span style="line-height:200%"><span new="" roman="" times=""> Clinical isolates of fluconazole<span style="background:white"><span style="color:black">-resistant </span></span><i>C. tropicalis</i> were identified using the CLSI guidelines and the World Health Organization&#39;s WHONET software. The inhibitory effect of carvacrol on planktonic cells was assessed by determining the minimum inhibitory concentration (MIC) and time-kill profile. The inhibitory effect of carvacrol on hyphal growth was studied by using light field <span style="color:black">microscopy.</span></span></span><span style="line-height:200%"><span new="" roman="" times=""><span style="color:black"></span></span></span></span></span></span><br> <span style="line-height:200%"><span style="unicode-bidi:embed"><span calibri=""><b><span style="line-height:200%"><span new="" roman="" times="">Results:</span></span></b><span style="line-height:200%"><span new="" roman="" times=""> The findings indicated that 50% of clinical isolates of </span></span><i><span style="line-height:200%"><span new="" roman="" times="">C. tropicalis</span></span></i> <span style="line-height:200%"><span new="" roman="" times="">were resistant to fluconazole. The MIC90 and MIC50 <span style="background:white"><span style="color:black">of carvacrol against&nbsp;</span></span>clinical isolates of fluconazole<span style="background:white"><span style="color:black">-susceptible and -resistant </span></span><i>C. tropicalis</i> <span style="background:white"><span style="color:black">were </span></span>25.00-300.00 &micro;g/ml and 12.50-100.00 &micro;g/ml, respectively<span style="background:white"><span style="color:black">. The </span></span>time-kill analysis</span></span><span style="background:white"><span style="line-height:200%"><span new="" roman="" times=""><span style="color:black"> indicated that carvacrol exhibited fungicidal activity against&nbsp;the </span></span></span></span><span style="line-height:200%"><span new="" roman="" times="">fluconazole<span style="background:white"><span style="color:black">-susceptible and -resistant </span></span><i>C. tropicalis </i>isolates <span style="background:white"><span style="color:black">2-48 hours after exposure. Moreover, </span></span>planktonic and hyphal growth of the isolates decreased significantly after exposure to </span></span><span style="background:white"><span style="line-height:200%"><span new="" roman="" times=""><span style="color:black">carvacrol</span></span></span></span><span style="line-height:200%"><span new="" roman="" times="">. </span></span><span style="line-height:200%"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span><br> <b><span style="line-height:107%"><span new="" roman="" times="">Conclusion:</span></span></b><span style="line-height:107%"><span new="" roman="" times=""> The findings revealed that carvacrol exhibits inhibitory effects on the planktonic and hyphal cells of fluconazole<span style="background:white"><span style="color:black">-susceptible and -resistant </span></span></span></span><i><span style="line-height:107%"><span new="" roman="" times="">C. tropicalis</span></span></i><span style="line-height:107%"><span new="" roman="" times=""> isolates. Therefore, the antifungal potential of carvacrol as a natural antifungal could be further exploited for the treatment of resistant <i>C. tropicalis</i> infections</span></span></span></span></div> Carvacrol,Candida tropicalis,Fluconazole, 27 34 http://mlj.goums.ac.ir/browse.php?a_code=A-10-1034-1&slc_lang=en&sid=1 Marziyeh Erfaninia iauyasouj@gmail.com 100319475328460031954 100319475328460031954 No Department of Microbiology, Yasooj Branch, Islamic Azad University, Yasooj, Iran Fahimeh Alizadeh mnalizadeh@yahoo.com 100319475328460031955 100319475328460031955 Yes Department of Microbiology, Yasooj Branch, Islamic Azad University, Yasooj, Iran