Medical Laboratory Journal
Medical Laboratory Journal
mljgoums
Medical Sciences
http://mlj.goums.ac.ir
1
admin
2538-4449
10.52547/mlj
en
jalali
1400
6
1
gregorian
2021
9
1
15
5
online
1
fulltext
en
Development of a New Framework for Health Assessment in Patients with Coronary Artery Disease by using miRNA-197 in Adults
پزشکی مولکولی
Molecular Medicine
تحقيقي
Original Paper
<strong>Background and objectives: </strong>Coronary artery disease (CAD) refers to stenosis or obstruction of coronary artery due to atherosclerosis or clotting. The aim of this study was to evaluate possible association of serum miRNA-197 (miR-197) expression as a biomarker for CAD diagnosis.<br>
<strong>Methods:</strong> In this study, 100 patients with CAD who had angiography and vascular transplantation were selected. Expression of miR-197 was evaluated using real-time RT-PCR technique and the SYBR Green method. The Pearson's correlation coefficient was used to determine relationship of miR-197 expression and severity of coronary artery disease. The t-test was used to determine significance of expression of miR-197 in the study groups. All statistical analyses were carried out in SPSS 16 and at significance of 0.05.<br>
<strong>Results:</strong> The results showed a <strong>direct relationship </strong>between miR-197 expression and CAD severity. The relative expression of miR-197 in the CAD patients was significantly higher than that in control subjects (P<0.004).<br>
<strong>Conclusion:</strong> It seems that miR-197 can be considered as an indicator of coronary endothelial cell function. This microRNA could be used as a biomarker for CAD prognosis and treatment progression.<span dir="RTL"></span>
MIRN197 microRNA,human,Coronary Artery Disease,U6 small nuclear RNA,
27
31
http://mlj.goums.ac.ir/browse.php?a_code=A-10-941-1&slc_lang=en&sid=1
Shahram
Zehtabian
rosagol1343@gmail.com
100319475328460021041
100319475328460021041
No
Department of Biology, Faculty of Biological Science, Islamic Azad University, North Tehran Branch, Tehran, Iran
reza
alibakhshi
ralibakhshiy@gmail.com
100319475328460021042
100319475328460021042
Yes
kermanshah university of medical sciences
Seyed Yousef
Seyedena
y.seyedena@yahoo.com
100319475328460021043
100319475328460021043
No
Department of biology, faculty of Biological science, Islamic Azad University, North Tehran Branch, Tehran, Iran
Ali-Reza
Rai
alireza_rai@yahoo.com
100319475328460021044
100319475328460021044
No
kermanshah university of medical sciences