Medical Laboratory Journal
Medical Laboratory Journal
mljgoums
Medical Sciences
http://mlj.goums.ac.ir
1
admin
2538-4449
10.52547/mlj
en
jalali
1399
4
1
gregorian
2020
7
1
14
4
online
1
fulltext
en
Effect of Eight Weeks of Aerobic Exercise and Vitamin D Supplementation on 8-hydroxy-2'-deoxyguanosine and O6-methylguanine DNA methyltransferase in Lung of Rats Poisoned with Hydrogen Peroxide
فیزیولوژی ورزشی
Sport Physiology
تحقيقي
Original Paper
<div><strong>Background and Objectives: </strong>Prolonged exercise can reduce physiological capacities and cause DNA damage by inducing oxidative stress and inflammatory responses. Aerobic exercise reduces the risk of cancer by activating DNA repair enzymes and reducing oxidative stress. The aim of the present study was to investigate effects of eight weeks of aerobic exercise with and without vitamin D supplementation on DNA damage.<br>
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<strong> Methods: </strong>Forty-eight adult male rats were randomly divided into six groups: control (C), H<sub>2</sub>O<sub>2</sub> (H), H<sub>2</sub>O<sub>2 </sub>and vitamin D (HD), H<sub>2</sub>O<sub>2</sub> and exercise (HE), H<sub>2</sub>O<sub>2,</sub>, vitamin D and exercise (HDE), and dimethyl sulfoxide. Cancer was stimulated through intraperitoneal injection of H<sub>2</sub>O<sub>2 </sub>(2 mmol/kg). Animals in groups HE and HDE ran on treadmill for eight weeks. Concentration of 8-hydroxy-2'-deoxyguanosine (8-OHdG) and O<sup>6</sup>-methylguanine DNA methyltransferase (MGMT) was measured by enzyme-linked immunosorbent assay. Statistical analysis of data was carried out using SPSS 22 at significance level of 0.05.<br>
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<strong> Results</strong><strong>:</strong> Vitamin D supplementation significantly lowered the level of 8-OHdG expression compared to the control group (P=0.0001). The 8-OHdG expression in the exercise group was slightly lower than control group (P=0.063). Combination of exercise and vitamin D supplementation had no significant effect on expression of 8-OHdG (P=0.281). Both exercise and vitamin D supplementation significantly increased MGMT expression compared to the control group (P=0.0001 and P=0.040). However, combination of exercise and vitamin D supplementation had no significant effect on MGMT expression (P=0.326).<br>
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<strong> Conclusion:</strong> The results showed that aerobic exercise and vitamin D supplementation can have protective effects against DNA damage, possibly by increasing antioxidant capacity and DNA repair.</div>
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DNA, 8-OHdG, 6-Methyl Guanine, Vitamin D, Exercise
31
37
http://mlj.goums.ac.ir/browse.php?a_code=A-10-694-2&slc_lang=en&sid=1
seyedeh tahereh
haeri
Tahaereh.haeri@yahoo.com
100319475328460018255
100319475328460018255
No
Department of Exercise Physiology, Central Tehran Branch,
mohammad ali
azarbayjani
mail: m_azarbayjani@iauctb.ac.ir
100319475328460018256
100319475328460018256
Yes
Department of Exercise Physiology, Central Tehran Branch, I
Maghsoud
peeri
m.peeri@iauctb.ac.ir
100319475328460018257
100319475328460018257
No
Department of Exercise Physiology, Central Tehran Branch,