Medical Laboratory Journal
Medical Laboratory Journal
mljgoums
Medical Sciences
http://mlj.goums.ac.ir
1
admin
2538-4449
10.52547/mlj
en
jalali
1400
10
1
gregorian
2022
1
1
16
1
online
1
fulltext
en
Effect of Exercise Training and Atorvastatin Supplementation on Beclin1, LC3-I and LC3-П Expression in Old Diabetic Rats
فیزیولوژی ورزشی
Sport Physiology
تحقيقي
Original Paper
<strong>Background and objectives: </strong>Programmed autophagy is a genetically and evolutionarily conserved process that destroys long-lived cellular proteins and organelles. This study aimed to investigate effects of continuous and interval exercise training with or without atorvastatin supplementation on Beclin1, LC3-I and LC3-П expression in old rats with type 2 diabetes<span dir="RTL">.</span><br>
<strong>Methods</strong>: Sixty three male Wistar rats were divided into eight groups. Continuous exercise was performed at a speed of 15-29 m/min for 5-22 minutes. Interval exercise program consisted of six 2.5-minute sets that included a four-minute rest period between each set. The rats in the supplementation groups also received 20 mg/kg body weight atorvastatin daily via intraperitoneal injection. At the end of the training period, the expression of Beclin1, LC3-I and LC3-П in soleus muscle was measured by RT-PCR. One-way ANOVA was used for data analysis at statistical significance of 0.05<span dir="RTL">.</span><br>
<strong>Results</strong>: The results showed that both exercise trainings with or without atorvastatin significantly reduced LC3I, LC3-II and Beclin1 compared with the diabetic control group (P<0.05)<span dir="RTL">.</span> In addition, the effects of the trainings and atorvastatin supplement did not differ significantly (P>0.05)<span dir="RTL">.</span><br>
<strong>Conclusion</strong>: The results indicate that continuous and interval exercise program alone and combined with atorvastatin supplementation could significantly reduce LC3-1, LC3-II and Beclin1 level in soleus muscle of old diabetic rats.
Autophagy,Diabetes Mellitus, Type 2,Atorvastatin,
40
47
http://mlj.goums.ac.ir/browse.php?a_code=A-10-233-8&slc_lang=en&sid=1
farnaz
zanghaneh
zanganeh.farnaz@yahoo.com
100319475328460021406
100319475328460021406
No
Department of Exercise Physiology, Aliabad Katul Branch, Islamic Azad University, Aliabad Katul, Iran
parvin
farzanegi
parvin.farzanegi@gmail.com
100319475328460021407
100319475328460021407
Yes
Department of Exercise Physiology, Sari Branch, Islamic Azad University, Sari, Iran
habib
asgharpour
100319475328460021408
100319475328460021408
No
Department of Exercise Physiology, Aliabad Katul Branch, Islamic Azad University, Aliabad Katul, Iran