Medical Laboratory Journal

fa Identification of Pathogenic Leptospiral Serovars by Detection of the ompl37 Gene Using PCR علوم آزمایشگاهی Laboratory Sciences تحقيقي Original Paper <div>ABSTRACT             Background and Objectives: Leptospirosis is a widespread zoonotic disease that is transmitted directly or indirectly from animals to humans. Humans mainly acquire pathogenic leptospires through mucosal or percutaneous exposure to environment contaminated with urine from an infected animal. We aimed to identify pathogenic leptospiral serovars by detection of the ompL37 gene using polymerase chain reaction (PCR).             Methods: Sixteen pathogenic leptospiral serovars and a saprophytic serovar, L. biflexa were cultured in modified semisolid Ellinghausen-McCullough-Johnson-Harris medium containing 5% rabbit serum. Genomic DNA extraction was done using the phenol-chlorophorm method. The ompL37 gene was amplified using specific primers. PCR products were analyzed by agarose gel electrophoresis.             Results: The ompL37 gene was amplified only in the pathogenic leptospiral serovars. We detected no amplified fragment for the saprophytic serovar. Conclusion: Leptospirosis may be confused with other infectious diseases, and therefore, its early and accurate diagnosis is crucial. We showed that molecular detection of pathogenic leptospires based on the ompL37 gene could be used for laboratory diagnosis of leptospirosis.             Keywords: Leptospirosis, PCR, ompl37 Gene, Pathogenic Leptospires. </div> Leptospirosis, PCR, ompl37 Gene, Pathogenic Leptospires. 33 36 http://mlj.goums.ac.ir/browse.php?a_code=A-10-617-3&slc_lang=fa&sid=1 Elaheh Rezaei 100319475328460014970 100319475328460014970 No Department of Microbiology, Razi Vaccine & Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran Pejvak Khaki P.khaki@rvsri.ac.ir 100319475328460014971 100319475328460014971 Yes Department of Microbiology, Razi Vaccine & Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran Soheila Moradi Bidhendi 100319475328460014972 100319475328460014972 No Department of Microbiology, Razi Vaccine & Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran Mojtaba Noofeli 100319475328460014973 100319475328460014973 No Department of human bacterial vaccines, Razi Vaccine & Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran