Medical Laboratory Journal
Medical Laboratory Journal
mljgoums
Medical Sciences
http://mlj.goums.ac.ir
1
admin
2538-4449
2538-4449
10.61186/mlj
en
jalali
1398
10
1
gregorian
2020
1
1
14
1
online
1
fulltext
en
Probable Role of Bilophila wadsworthia in Appendicle Infection
ميکروب شناسي
Microbiology
تحقيقي
Original Paper
<div><strong>ABSTRACT</strong><br>
<strong> Background and Objective: </strong><em>Bilophlia</em> spp. are gram-negative, pleomorphic rod, obligate anaerobe, oxidase-negative, catalase-positive and non-motile bacteria. <em>B. wadsworthia</em> is type species of genus <em>Bilophila</em> with the additional characteristic of urea hydrolysis<em>. B. wadsworthia</em> can be found in <u>a</u> variety of anaerobe infection<u>s</u>, <u>particularly</u> appendicitis and intra-abdominal infection that <u>are</u> <u>considered</u> as important opportunistic pathogen<u>s</u>.<br>
<strong> Methods</strong>: This study<u> was designed to identify</u> <em>Bilophila </em>spp. in clinical specimens by culture and PCR. We examined 91 DNA sample<u>s</u> extracted from infected appendix tissues with specific primers.<br>
<strong> Results: </strong>Data <u>showed</u> that <em>Bilophila</em> spp. DNA existence in 53.85% (n=49) provided appendiceal tissue.<br>
<strong> Conclusion: </strong><u>The</u> pathological and molecular examination of infected appendiceal tissues <u>revealed</u> that <em>B. wadsworthia </em><u>is able </u>to act as <u>the</u> primary cause of significant lesions in the appendicle tissues.<br>
<strong> Key words</strong>: <em>Bilophila</em> spp., <u>A</u>ppendectomy, <u>A</u>ppendicle specimens, PCR, Nucleotide sequencing</div>
Bilophila spp., appendectomy, appendicle specimens, PCR, Nucleotide sequencing
29
35
http://mlj.goums.ac.ir/browse.php?a_code=A-10-622-1&slc_lang=en&sid=1
Mohammad
Tabatabaei
mtabatabaei2003@yahoo.co.uk
100319475328460017172
100319475328460017172
Yes
Assoociated Professor Microbiologist, Faculty of Veterinary Medicine, Shiraz University, Shiraz, Iran
Aslam
Dehvari
dehvari87@gmail.com
100319475328460017173
100319475328460017173
No
M.Sc. student in bacteriology, Faculty of Veterinary Medicine, Shiraz University, Shiraz, Iran
Bita
Geramizadeh
geramib@gmail.com
100319475328460017174
100319475328460017174
No
Professor, Pathologist, Shiraz Transplant Research Center, Shiraz University of Medical SciencesShiraz, Iran
Mohammad Hadi
Niakan
hadiniakan@yahoo.com
100319475328460017175
100319475328460017175
No
Surgeon, Trauma Research Center, Shiraz University of Medical Sciences, Shiraz, Iran,