Medical Laboratory Journal
Medical Laboratory Journal
mljgoums
Medical Sciences
http://mlj.goums.ac.ir
1
admin
2538-4449
10.52547/mlj
en
jalali
1397
2
1
gregorian
2018
5
1
12
3
online
1
fulltext
fa
Isolation and Molecular Identification of Keratinase-Producing Bacteria from the Sludge of Qeshm Island
تحقيقي
Original Paper
<div>
<div><strong>ABSTRACT</strong><br>
<strong> Background and Objectives:</strong> Keratinase is an enzyme commonly used in the production of detergents, cosmetics, drugs, leather, and other industries. Considering the high cost of traditional methods for decomposition of feather, hair, hooves, nails, and wool that contain high levels of keratin, their biodegradation with keratinase-producing bacteria can be a valuable solution. The present study aimed for isolation and molecular identification of keratinase-producing bacteria in Qeshm Island and Peyposht village in Iran.<br>
<strong>Methods:</strong> Water and sludge samples from the Qeshm Island and Peyposht village were collected. The bacteria isolates were screened for keratinase production using the Lowry method. Effect of pH and temperature was assessed on the production of keratinase and on the growth of the isolates. Colony-polymerase chain reaction was used for molecular identification of the isolates.<span dir="RTL"></span><br>
<strong>Results:</strong><em> Bacillus berevis</em> and <em>Enterobacter cloacae</em> were isolated in this study. Keratinase production in <em>B. berevis</em> was highest at pH 7.5 and 35 °C. In addition, the highest level of enzyme production by <em>E. cloacae</em> was observed at pH 7 and 37 °C.<br>
<strong>Conclusion:</strong> It seems that the bacterial strains isolated from sludge in the study area have relatively favorable keratinase production capacity.<span dir="RTL"></span><br>
<strong>Keywords:</strong> Bacteria, Colony PCR, Identification, Keratinolytic protein, Sewage.</div>
<br>
</div>
Bacteria, Colony PCR, Identification, Keratinolytic protein, Sewage
34
40
http://mlj.goums.ac.ir/browse.php?a_code=A-10-1-510&slc_lang=fa&sid=1
Maedeh
Kiani Abri
100319475328460014305
100319475328460014305
No
Department of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran
Monir
Doudi
Doudi@iaufala.ac.ir
100319475328460014306
100319475328460014306
Yes
Department of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran
Ali Mohammad
Ahadi
100319475328460014307
100319475328460014307
No
Department of Genetics, Shahrekord University Branch, Shahrekord, Iran