This paper should be cited as: SoltanDallal, MM. Rahbar, M. Douraghi, M. RahimiForooshani, A. Khan Babaei, GT. Mobarhan, M. Ghasemi, F.
Antibiotic Susceptibility of Pseudomonas Aeruginosa Isolated from Cystic Fibrosis Patients
SoltanDallal, MM. (PhD)1 Rahbar, M. (PhD)2 Douraghi, M. (PhD)3 RahimiForooshani, A. (PhD)4 Khan Babaei, GT. (PhD)5 Mobarhan, M. (MSc)6 Ghasemi, F. (MSc)6
1. Professor of Microbiology, Food Microbiology Research Center, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
2. Professor of Microbiology, Reference Laboratory of Health, Ministry of Health and Medical Education, Tehran, Iran
3. Assistant Professor of Microbiology, Food Microbiology Research Center, Tehran University of Medical Sciences, Tehran, Iran
4. Associate Professor of Epidemiology and Biostatics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
5. Associate Professor of Pediatrics, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
6. MSc of Bacteriology, Department of Microbiology, Tarbiat Modares University, Tehran, Iran
7. MSc of Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
Abstract
Background and Objective: Cystic fibrosis (CF) is an autosomal recessive genetic disease and Pseudomonas aeruginosa is one of the most common bacteria colonized in CF patients. Growing resistance of this bacterium to antibiotics now a day is a challenge of controlling infection in CF patient. In this study colonization of CF patients with Pseudomonas aeruginosa and antibiotic susceptibility pattern of isolated strains were examined.
Material and Methods: From 100 CF patients, during a year, sputum and bronchial swabs were collected. After culturing the samples, some of them were reported as Pseudomonas aeruginosa using biochemical tests. Mucoid strains of Pseudomonas aeruginosa were identified the same as non-producing alginate strains while for catching single pure colony,repeated passage was used. For determining antibiotic resistance of Pseudomonas aeruginosa to some antimicrobial agents Kirby-Bauer method based on CLSI was used.
Results: Of 100 samples, 40 (40%) were positive for Pseudompnas aeruginosa. The prevalence of P.aeruginosa was 23.8,36.84 and 80% at the age of 1-3, 4-12 and 13, respectively.
Conclusion: Statistically, there is a significant difference between age and contracting with Pseudomonas aeruginosa in that the higher the age the more colonization with Pseudomonas aeruginosa.
Key words:Pseudomonas aeruginosa, Cystic Fibrosis, Drug Resistance
Corresponding Author: Ghasemi, F.
Email: ghasemi_ghasemi@yahoo.com
Received: 6 Jul
2013
Revised: 6 Jun 2014
Accepted: 7 Jul 2014