This paper should be cited as: Ebrahimipour, GH. Moradi, A. Karkhane, M. Marzban, AR.

Antibiotics and Heavy Metal Resistance of Three Strains of Pseudomonas Aeruginosa Isolated from Different Ecological Areas

Ebrahimipour, GH. (PhD)¹, Moradi, A. (MSc)², Karkhane, M. (MSc)³, Marzban, AR. (MSc)⁴

1. Associate Professor of Microbiology, Department of Microbiology, School of Biological Sciences, Shahid Beheshti University, Tehran, Iran

2. PhD Student of Microbiology, Department of Microbiology, School of Biological Sciences, Shahid Beheshti University, Tehran, Iran

3. MSc of Microbiology, Gastroenterology and Liver Diseases Research Center, Shahid Beheshti University of Medical sciences, Tehran, Iran

4. PhD Student of Pharmaceutical Biotechnology, Biotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran

Abstract

Bachground and Objective: most of environmental microorganisms have the genes resistance to antibiotics and metals. The aim of the current study was to survey resistance pattern to some antibiotics and heavy metals in three pseudomonas aeruginosa isolated from different ecological areas.

Material and Methods: first, the isolates were identified by biochemical methods and phylogenetic analysis. Then, the evaluation of antibiotic resistance was conducted by disc diffusion and that of Heavy metal resistant by agar dilution, in a range of 50-500 µg/ml.

Results: The results showed that all three isolates were resistant to beta lactam antibiotics. Although these isolates were highly resistant to heavy metals, no relationship was observed between ecological sources and the resistance pattern in ICT1 and Abt2 strains. However, strain Q isolated from digestive system of ParmacellaIberica showed high resistance to antibiotics and low resistance to heavy metals.

Conclusion: given that environmental bacteria have a high potentiality for carrying resistance genes and this can be an advantage environmentally, they could be used to remove heavy metals from polluted areas. On the other hand, resistance genes medically are a concern due to probability of transferring to pathogen strains.

Keywords: Antibiotic Resistance, Heavy Metal Resistance, Pseudomonas Aeruginosa

Corresponding Author: Marzban, AR.

Email: Marzbana901@mums.ac.ir

Received: 25 Jun 2014
Revised: 27 Sep 2014
Accepted: 7 Oct 2014