This paper should be cited as: Mahmoudjanlou, H. Ghazisaeedi, k. Moradi, A. Shakeri,
F. Mansoor Samae, N. Medical Laboratory Journal, Autumn- Winter 2012, Vol.6,
No.2.[Article in Persian]
Minimum Inhibitory Concentration of
Cefotaxime by E-test Method on Klebsiella in Gorgan
Mahmoudjanlou, H.(MSc)*1, Ghazisaeedi, k.(PhD)2,
Moradi, A. (PhD)3, Shakeri, F.(Msc)4, Babaii Koochaksarii, M. (MSc)5, Mansoor
Samae, N.( PhD)6
1 MSc of Microbiology,Golestan
University of Medical Sciences
2 Professor of Microbiology , Microbiology Department,
Golestan University of Medical Sciences
3 Professor of Virology, Microbiology
Department, Golestan University of Medical Sciences
4 MSc of of Microbiology,Golestan
University of Medical Science
5 MSc of Microbiology, Tonekabon Islamic Azad
University
6 Assistant Professor of Human Genetic,
Golestan University of Medical Science
Abstract
Background
and objectives: the increasing use of
antibiotics, especially the third
generation cephalosporins, is an
important factor in the spread of antibiotic resistance in bacteria. The main reason
for the development of resistance phenotype such as Extended Spectrum Beta Lactamas (ESBL) is the extensive use of
broad-spectrum cephalosporins. In phenotypic survey, the Phenotyping confirmatory
test and the minimum inhibitory
concentration (MIC) are used.
In this study, the prevalence of
the isolates resistant to third generation cephalosporin (cefotaxime) was determined based on MIC.
Material
and Methods: form September 2010 to September 2011, 75 isolates of
Klebsiella pneumoniae were collected from the infections of inpatients and
outpatients, referred to state and private laboratories of Gorgan. For all of
the Klebsiella pneumoniae strains, MIC determination using E-test
(company Liofilcheme-Italy) was performed.
Results:
According to the MIC results, 26 samples (34.6%) are resistant to cefotaxime;
22 isolates are completely resistant to concentration of 256μg.
Conclusion: Because
of the importance of risk of becoming ESBL, further studies are needed to
clarify the ESBL in the region.
Keywords:
ESBL, MIC, Klebsiella pneumoniae, Cephalosporin
Corresponding author:
Mahmoudjanlou, H. E- mail: janloo_h@yahoo.com
Received1May2012 Revised 6 Oct 2012 Accepted 8 Oct 2012