This paper should be cited as: Keikha, N. Ayatollahi Mousavi, SA. Nakhaei, AR. Amanloo, S. Shahidi Benjar, GH. Amiri, S.

In Vitro Comparison of MIC Crude extracts of Active Actinomycetes Isolates with Terbinafine, Griseofulvin Ketoconazole and Fluconazole against Microsporum canis, Microsporum gypseum and Trichophyton mentagrophytes

Keikha, N. (MSc)1, Ayatollahi Mousavi, SA. (PhD)2, Nakhaei, AR. (PhD)3, Amanloo, S. (MSc)4, Shahidi Benjar, GH. (PhD)5, Amiri, S. (MSc)6

1. MSc of Medical Mycology, Department of Laboratory Sciences, Zahedan University of Medical Sciences, Zahedan, Iran

2. Associate Professor of Medical Mycology, Deptartment of Medical Mycology & Parasitology, Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran

3. Associate Professor of Biochemistry, Department of Biochemistry, Zahedan University of Medical Sciences, Zahedan, Iran

4. MSc of Medical Mycology, Department of Medical Mycology & Parasitology, Zabol University of Medical Science, Zabol, Iran

5. Professor of Plant Pathology, Faculty of Agricultural Engineering, Shahid Bahonar University of Kerman, Kerman, Iran

6. MA in State Management, Zahedan Economic and Finance Organization, Zahedan, Iran

Abstract

Background and Objective: Dermatophytes are the fungi that have the ability to attack the keratinized tissues such as the skin, hair and nails. Infections caused by these organisms are named dermatophytosis. We aimed to compare Minimum inhibitory concentration (MIC) of Crude extracts of Active Actinomycete Isolates with Terbinafine, Griseofulvin, Ketoconazole and Fluconazole Drugs against Microsporum Canis, Microsporum gypseum and Trichophyton mentagrophytes.

Material and Methods: In this experimental study, in order to find MIC by actionmycete, 100 isolates were studied and then crude extracts of the active actinomycete isolates were prepared in sterile conditions. Finally, the crude extracts obtained at different concentrations were used to obtain the MIC of Microsporum Canis, Microsporum gypseum and Trichophyton mentagrophytes. Moreover, various concentrations of the drugs such as terbinafine, griseofulvin, ketoconazole and fluconazole in solvent Dimethyl sulfoxide (DMSO) were prepared and their growth inhibitory effect was evaluated and then compared with the results obtained from the crude extract of active actinomycete isolates.

Results: the crude extracts obtained from active Actioiomycetes isolates and the drugs such as terbinafine, griseofulvin, ketoconazole and fluconazole, in a dose-dependent manner, could inhibit the growth of Microsporum Canis, Microsporum gypseum and Trichophyton Mentagrophytes.

Conclusion: compared to MIC of Crude extract of active actinomycete isolates, Terbinafine has a significant effect on the growth inhibition in all of the fungal Dermatophytes and then griseofulvin, ketoconazole and fluconazole are in the next rank, respectively.

Keywords: Actinomycetes Crude Extract, Microsporum Canis, Microsporum Gypseum, Terbinafine

Corresponding Author: Keikha, N.

Email: Nasserkeikha@yahoo.com

Received: 4 Nov 2013
Revised: 30 Jul 2014
Accepted: 3 Aug 2014

This paper should be cited as: Keikha N, Nakhaei AR, Ayatollahi Mousavi SA, Amanloo S, Shahidi Benjar GH, Amiri S. [In Vitro Comparison of MIC Crude extracts of Active Actinomycetes Isolates with Terbinafine, Griseofulvin Ketoconazole and Fluconazole against Microsporum canis, Microsporum gypseum and Trichophyton mentagrophytes]. mljgoums. 2015; 9(1): 53-60[Article in Persian]