This paper should be cited as: Mahmoudjanlou, H. Ghazisaeedi, K. Shakeri, F. Ghaemi, EA.
Prevalence of Klebsiella Pneumoniae in Panje Azar Hospital of Gorgan, Iran, 2011
Mahmoudjanlou, H. (MSc)1 Ghazisaeedi, K. (PhD)2 Shakeri, F. (MSc)3 Ghaemi, EA. (PhD)4
1. Msc of Microbiology, Golestan University of Medical Science, Gorgan, Iran
2. Professor of Microbiology, Department of Microbiology, Golestan University of Medical Science, Gorgan, Iran
3. MSc of Microbiology, Payame Noor University, Gorgan, Iran
4. Professor of Microbiology, Department of Microbiology, Golestan University of Medical Science, Gorgan, Iran
Abstract
Background and Objective: Klebsiella pneumoniae is one of the agents causing nosocomial infection; therefore, we decided to report the prevalence of Klebsiella pneumoniae caused infection.
Material and methods: The frequency of Klebsiella in culture media samples of Panje Azar hospital was studied in 2011-2012. After determination of the species with biochemical methods and determination of resistance to third generation cephalosporins, the existence of responsible genes for this resistance was investigated using specific primers. The PCR product for CTX-M gene was sequenced.
Results: During the study, 70 isolates of Klebsiella were isolated in that 51 (72.8%) related to three months of November, December and January. Except for the one related to November, other ESBL cases belonged to these three months. Based on molecular investigation of ESBL genes, these isolates at least were in 3 types and had a high frequency in Internal, female and Emergency wards.
Conclusion: The present report implied a sudden prevalence of Klebsiella pneumoniae that detected and controlled by a correct monitoring.
Keyword: Klebsiella Pneumoniae, ESBL, CTX-M
Corresponding Author: Ghaemi, EA.
Email: eghaemi@yahoo.com
Received: 24
Apr 2013
Revised: 18 Jun 2013
Accepted: 25 May 2013