This paper should be cited as:Moulana Z, Ghasem Pour M, Elmi MM, Baghban Shaker P.[The
Frequency of Streptococcus Mutans and
Lactobacillus spp. in 3-5-year- Old Children with and without
Dental Caries]. MLJ. 2013; 7(1):
The Frequency of Streptococcus Mutans and Lactobacillus spp. in 3-5-year- Old Children with and without Dental Caries
Moulana, Z.
(MSc)*1, Ghasem Pour, M. (PhD)2,
Asghar
Pour, F. (MSc)3, Elmi, MM. (PhD)4,
Baghban Shaker, P.(PhD)5
1. MSc of Microbiology, Infectious Diseases & Tropical Medicine
Research Center, and paramedical department, Babol University of Medical
Sciences, Babol, Iran
2. Assistant Professor of Pedodontics, Department of Pedodontics,
Faculty of Dentistry, Babol University of Medical Sciences , Babol, Iran
3. MSc of Biochemistry, Paramedical
department, Babol University of Medical Sciences , Babol, Iran
4. Phd of Biophysics,
Cellular and Molecular biology research center , and paramedical department,
Babol University of Medical Sciences, Babol, Iran
5. Doctor of Dental Surgeon, Faculty of Dentistry, Babol University of Medical Sciences
, Babol, Iran
Abstract
Background
and Objective:The high occurrence of early childhood caries (ECC) is one of the
most common problems in children dentistry.
Streptococcusmutans and Lactobacilli.spp are closely
associated with the development of early childhood caries (ECC).Therefore, the
aim of this study was to investigate the frequency of Streptococcus mutans and
Lactobacilli.spp in 3-5 –year- old children with and
without dental caries.
Material
and Methods: This case-control study was conducted on 60 children aged 3
-5-years, without any history of systemic disease, who had not received any
antibiotic therapy and fluoride usage during the last month. The Cases were divided into three groups of
early childhood caries, usual caries and caries-free.
The infected dentin samples were collected from cervical and proximal in ECC
and usual caries group, respectively. Also in all the three groups, the samples
of dental plaque from buccal surfaces were collected and
immediately immersed into Brain Heart Infusion (BHI) broth medium. After
that the diluted sample was plated onto MitisSalivarius agar (Difco)
for detecting Streptococcus mutans and Rogosaagar (Difco) for detecting Lactobacilli.spp.
Data were analyzed by Chi- square and ANOVA.
Results: of the samples
taken from dental plaque, S. mutans is observed in 90% of ECC, proxymol carries
in 80% and no caries in 25% of the casest. Based on the results, the
presence of S.mutans in the group of caries is significantly higher than that
of without caries, but there is no significant difference between the two
groups of caries. In addition, Lactobacill.spp is isolated from 60% of samples,
but there is no significant difference between two groups of caries and the
group of without caries. The average of
decayed, missing and filled teeth (DMFT) index in the group with early
childhood caries (6.95±1.572) and usual caries (5.80±1.105) is different significantly. Pearson
correlation test shows that there is a significant relationship between caries
experience and the amount of S.mutans, but there isn’t this relationship to
Lactobacill.spp. Conclusion: The finding shows that the children with early
decay have the higher level of S.mutans in their dental plaque. The most common strain isolated from dentin, without consideration
of their location, is S.mutans.
Key words: Early dental care,Dental caries,DMFT index,Streptococcus mutans,
Lactobacilli.spp.
CorrespondingAuthor:
Moulana Z
Email:zmoulana@yahoo.com
Received 14 Apr 2012Revised 10 Dec
2012Accepted 11 Dec 2012