This paper should be cited as: Jamshidi, A, Mousavi Ghahfarrokhi, M, Gharaei, A, Ebrahimzadeh, A, Ansari Moghadam, A, Mohammadi, S, Ansari Moghadam, A. (PhD)

Genotyping of C and F Regions of Plasmodium Falciparum EBA-175 in South-East of Iran

Jamshidi, A. (MSc)*1, Mousavi Ghahfarrokhi, M. (MSc)2, Gharaei, A. (MSc)3, Jaffari Modrek, M. (PhD)4, Jaffari Modrek, M. (PhD)5, Ansari Moghadam, A. (PhD)6,

1. MSc of Medical Parasitology, Zahedan University of Medical Sciences, Zahedan, Iran

2. MSc of Medical Parasitology, Zahedan University of Medical Sciences, Zahedan, Iran

3. MSc of Medical Parasitology, Zahedan University of Medical Sciences, Zahedan, Iran

4. Associate Professor of Parasitology, Department of Parasitology, Mycology and Medical Entomology Research Center, Infectious Diseases and Tropical Medicine, Zahedan University of Medical Sciences, Zahedan, Iran

5. Assistant Professor of Parasitology,Department of Parasitology, Mycology and Medical Entomology Research Center, Infectious Diseases and Tropical Medicine, Zahedan University of Medical Sciences, Zahedan, Iran

6. Associate Professor of Biostatistics and Epidemiology, Center for Health Promotion, Faculty of Health, Zahedan University of Medical Sciences, Zahedan, Iran

7. PhD Student of Cellular and Molecular, Faculty of Advanced Medical Technology, Golestan University of Medical Sciences, Gorgan, Iran

Abstract

Background and Objective: The Plasmodium falciparum EBA-175, via Sialic acid dependent glycophorin A, binds to red blood cells and thus plays a critical role in cell invasion. Some part of second allele in its gene encoding in FCR-3 (Section F) and CAMP (Section C) can be found. This study aimed to determine the prevalence of Plasmodium falciparum EBA-175KD alleles in southeastern Iran.

Material and Methods: In this cross-sectional study, using polymerase chain reaction Nest (Nested-PCR) with specific primers was used for the two parts of the EBA-175 gene to be proliferated.Ninety–four microscopic positive blood samples from individuals infected by Plasmodium falciparum were obtained from four different locations in southeastern Iran.

Results:Of 94 positive samples, 88 were antigen EBA-175KD.Genotype CAMP (714 bp) and FCR-3 (to 795 bp), respectively, in 31 (32.97 %) and 49 (52.12 %) were found. Eight samples have both FCR-3 and CAMP.

Conclusion:Both of EBA-175KD dimorphic genes were found. The frequency of FCR-3 allele was higher in the South East of Iran. Thus, this pattern can be considered in making Plasmodium falciparum vaccines for this area.

Key words: Plasmodium Falciparum; Erythrocyte Binding Antigen-175; South-East of Iran

Ansari Moghadam, A. (PhD)

Mohammadi, S. (MSc)

Corresponding Author: Jamshidi, A.

Email: a.jamshidi@zaums.ac.ir

Received: 13 Apr 2013 Revised: 26 Oct 2013 Accepted: 28 Oct 2013