This paper should be cited as: Kargar, M. Ebrahimi, E. Amini, J. Najafi, A. Kheirkhah, B.
Isolation, Identification and Antibiotic Resistance Profile of Listeria Monocytogenes Strains in Red Meats
Kargar, M. (PhD)1, Ebrahimi, E. (MSc)2, Amini, J. (MSc)3, Najafi, A. (MSc)4, Kheirkhah, B. (PhD)5
1. Associate Professor of Microbiology, Department of Microbiology, Islamic Azad University, Jahrom Branch, Iran
2. MSc of Microbiology, Department of Microbiology, Islamic Azad University, Jahrom Branch, Iran
3. PhD Student of Microbiology, Department of Microbiology, Islamic Azad University, Kerman Branch, Iran
4. PhD Student of Marine Microbiology, Department of Marine Microbiology, The Persian Gulf Marine Biotechnology Research Center, Bushehr University of Medical Sciences, Bushehr, Iran
5. Assistant Professor of Microbiology, Department of Microbiology, Islamic Azad University, Kerman Branch, Iran
Abstract
Background and Objective:Listeria monocytogenes is a bacterium transferred by foods and is the agent of many sporadic and epidemic diseases in humans. This study aimed to investigate the prevalence of L. monocytogenes and to determinine their antibiotic resistance profile in red meats.
Material and Methods:this cross-sectional study was performed on 400 red meat samples obtained from industrial slaughterhouses placed in Kerman, Iran. First, the samples were enriched with Simultaneous Enrichment Broth (SEB), and then plated onto Palcam agar and Tryptic Soy Broth Yeast Extract Broth (TSAYE). After identification of the isolates based on biochemical tests and PCR, the isolates were checked for their antibiotic resistance profile using disk Diffusion
Results:of 400 samples, 12 samples (3%) were contaminated with different species of Listeria. Using PCR, hly gene was recognized in eight samples (2%) of L. monocytogenes. Furthermore, there was a significant difference in isolation rate of lamb samples compared to cow ones. While all of the isolates were resistant to clindamycin, amikacin and chloramphenicol, they were sensitive to penicillin.
Conclusion:in spite of low rate of infection in red meat samples in Kerman city, due to high risk of Listeria contamination in red meats, we recommend applying a routine screening to identify this bacterium in our county.
Keywords:Listeria Monocytogenes, Hly Gene, Red Meat, Antibiotic, Kerman
Corresponding Author: Kargar, M.
Email: mkargar@jia.ac.ir
Received: 5 Apr
2014
Revised: 16 Sep 2014
Accepted: 21 Sep 2014