This paper should be cited as: Rajabi, M. Habibipour, R. Vesaghati Ezatpour, S. Vesaghati Ezatpour, S.
Antibacterial Effects of Extract of Alloe Vera and Stachys Inflata on Gram Positive and Negative Bacteria in In Vitro
Rajabi, M. (MSc)1, Habibipour, R. (PhD)2, Vesaghati Ezatpour, S. (BSc)3, Vesaghati Ezatpour, S. (BSc)4
1.MSc of Agricultural Biotechnology, Department of Medicinal Plants, Applied-Scientific University of Jihad-Agriculture, Hamedan, Iran
2.PhD of Mycology, Department of Microbiology, School of Basic Sciences,Islamic Azad University, Hamedan Branch, Hamedan, Iran
3.MSc Student of Medicinal Plants, Department of Medicinal Plants, Islamic Azad University, Abhar-Zanjan Branch, Zanjan, Iran
4 MSc Student of Medicinal Plants, Department of Medicinal Plants, Islamic Azad University, Abhar-Zanjan Branch, Zanjan, Iran
Abstract
Background and objective:Antimicrobial and antiviral effects of Alloe Vera and Stachys inflata have been proved. We aimed to investigate the effects of extract of Alloe Vera and Stachys inflate on the growth of some bacteria to take the place of chemical drugs.
Material and Methods:the extracts of both plants were prepared by maceration method; different concentrations were prepared using Mueller Hinton agar medium and tested by Disc diffusion. Furthermore,minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined by the Microdilution method.
Results: The effect of Alloe Vera extract was significant on Staphylococcus aureus. MIC and MBC of Aloe Vera extract on Bacillus subtilis were obtained in 230 and 410 mg/ml, respectively, which were 500 and 714 mg/ml for Haemophilus influenza. The extract of gel of Alloe Vera had no effect on Bacillus subtilis. The extract of leaf and gel of Alloe Vera had an inhibitory effect on Haemophilus influenza and Pseudomonads aeraginosa. The extract of Stachys inflata had an inhibitory effect on Haemophilus influenza, but it did not have any on Pseudomonads aeraginosa. The Extract of Stachys inflata had no effect on Bacillus subtilis, while showing significant effect on Staphylococcus. Among antibiotics, Ofloxacin had an effect on Haemophilus influenza. The extract of both plants did not show any effect on Klebsiella pneumonia.
Conclusion:Given the effect of Alloe Vera and Stachys inflata in laboratory conditions, we hope that these extracts will be used instead of chemical substances for making nutritional supplements to control human diseases.
Key words: Alloe Vera, Antibacterial, Stachys Inflata, Extract
Corresponding Author: Habibipour, R
Email: mjavadra@yahoo.com
Received: 11 Nov 2013
Revised: 5 Mar 2014
Accepted: 8 Mar 2014