This paper should be cited as: Monadi, M. Kargar, M. Naghiha, A. Najafi, A. Mohammadi, R.
Molecular Detection of
Salmonella Serovar Isolated from Eggs
Monadi, M. (MSc)1,
Kargar, M. (PhD)2, Naghiha,
A. (PhD)3, Najafi, A. (MSc)4, Mohammadi,
R. (MSc)5
1. MSc of Microbiology, Department of
Microbiology, Islamic Azad University, Jahrom Branch Jahrom, Iran
2. Associate Professor of
Microbiology, Department of Microbiology, Islamic Azad University, Jahrom Branch Jahrom, Iran
3. Assistant Professor of
Microbiology, Department of Animal Science, College of
Agriculture, Yasuj University, Yasuj, Iran
4. PhD Student of Marine Microbiology,
The Persian Gulf Marine Biotechnology Research Center,
Bushehr University of Medical Sciences, Bushehr, Iran
5. MSc of Microbiology, Herbal
Medicine Research Center, Faculty of Medicine, Yasuj
University of Medical Sciences, Yasuj, Iran
Abstract
Background and Objective: Salmonellosis is the most common type of food poisoning in
developed and developing countries that is caused by Salmonella
serotype. Hence, we aimed to identify the Salmonella serovars
in eggs obtained from Kohgiluyeh and Boyerahmad province and to evaluate antibiotic resistance
of the isolated strains.
Material and Methods: In this study, 210 eggs were collected from Kohgiluyeh
and Boyerahmad Province. The bacteria were isolated
and identified using biochemical tests. After extraction of genomic DNA,Salmonella gender,Salmonella
enteritidis and Salmonella typhimurium were investigated by invA,
fliC and sefA primers,
respectively, using Multiplex PCR method.
Results: of 210, 14 (6.66%)were contaminated with
Salmonella. Of these, 12 (5.71%) were Salmonella typhimurium
and 2 (0.95%) were related to Salmonella spp. None of the samples were
contaminated with Salmonella enteritidis. The
highest resistance was related to penicillin (100%) and neomycin (78.57%).
Conclusion: Salmonella typhimurium is the
predominant serovar causing contamination in the eggs
of this Province.Given the wide spread of antibiotic
resistance in different serotypes of Salmonella, we recommend avoiding
of indiscriminate use of antibiotics in livestock and poultry.
Keywords: Salmonella, Drug Resistance, Antibiotic, Multiplex PCR, Kohgiluyeh and Boyerahmad
Corresponding Author: Kargar, M.
Email: mkargar@jia.ac.ir
Received: 19
Oct 2013
Revised: 10 May 2014
Accepted: 14 May 2014
![Text Box: This paper should be cited as: Monadi M, Kargar M, Naghiha A, Najafi A, Mohammadi R. [Molecular Detection of Salmonella Serovar Isolated from Eggs]. mljgoums. 2015; 9(1): 17-24[Article in Persian]](New%20Microsoft%20Word%20Document_files/image001.png){kind=small}