This paper should be cited as: Shaffifar, M. Tabarraei, A. Sajadian, A. Fotouhi, F. Ghaemi, A
Immunologic Evaluation of DNA Vaccine Encoding Influenza Virus
M2 Gene in Type A- Influenza Mice Model
Shaffifar, M. (MSc)1,
Tabarraei, A. (PhD)2, Sajadian,
A. (MSc)3, Fotouhi, F. (PhD)4 Ghaemi,
A. (PhD)5
1. MSc of Medical
Virology, Infectious Diseases Research Center, Department of Microbiology, Golestan University of Medical Sciences, Gorgan, Iran
2. Associate
Professor of Medical Virology, Infectious Diseases Research Center, Department
of Microbiology, Golestan University of Medical
Sciences, Gorgan, Iran
3. MSc of Medical
Microbiology, Shefa Neuroscience Research
Centre, Tehran, Iran
Influenza Research
Lab, Pasteur Institute of Iran, Tehran, Iran
4. Assistant
Professor of Medical Virology, Influenza Research Lab, Pasteur Institute
of Iran, Tehran, Iran
5. Assistant
Professor of Medical Virology, Influenza Research Lab, Pasteur Institute
of Iran, Tehran, Iran
Abstract
Background and Objective:
The M2
gene expressing the conserved protein in influenza virus can be used to make a
single-dose vaccine with permanent immunity.
Material and
Methods: The mice were allocated to one case group immunized with
pcDNA3-M2 and two control groups with pcDNA and PBS,
in three dozes with interval of two weeks. Two weeks after the last injection,
Cellular immunity was analyzed by MTT lymphocyte proliferation, interferon
gamma (IFN-gamma) and interleukin 4 (IL-4) ratio assays. The remaining animals
were challenged with PR8 virus.
Results: The production rate
of IFN8 and IL4 in pcDNA - M2 group was higher than
that of control groups (P >0.0001).Given the results of lymphocyte
proliferation, Stimulation index (SI) in vaccinated mice was significantly
higher than that of control groups
(P<0.05).In comparison with mortality rate of 100% in control groups,
the animals Challenged with PR8 vaccine had a 50% fatal rate implying a high
protection level for this vaccine.
Conclusion: The pcDNA3-M2
Vaccine can be considered as a promising vaccine against influenza infections.
Keywords: Influenza Virus - Gene Vaccine, M2 Protein
Corresponding Author: Ghaemi, A.
Email: mkargar@jia.ac.ir
Received: 19
Oct 2013
Revised: 10 May 2014
Accepted: 14 May 2014