This paper should be cited as: Eramabadi, M. Tadayon,
K. Mosavari, N. Keshavarz,
R. Banihashemi, R.
Identification of Mycobacterium Tuberculosis Complex, Using
Molecular Methods
Eramabadi, M.
(MSc) 1,Tadayon, K. (PhD)*2, Mosavari, N. (PhD)3, Keshavarz, R. (DVM)4, Banihashemi,
R. (MSc)5, Ghaderi, Gh.
(PhD)6, Sekhavati, M (BSc)7, Ahmadi,
M. (MSc)8, Eramabadi, P. (BSc)9, Khodaverdi
Daryan, E. (MSc)10, Ya Haghi, E. (MSc)11, Mir Sharafodin,
H. (MSc)12
1. MSc
of Microbiology, Islamic Azad University, Karaj Branch, Iran
2.
Assistant Professor of Microbiology, Razi Vaccine
& Serum Research Institute, Karaj, Iran
3.
Assistant Professor of Microbiology, Razi Vaccine
& Serum Research Institute, Karaj, Iran
4.
Doctor of veterinary medicine, Razi Vaccine &
Serum Research Institute, Karaj, Iran
5. MSc
of Immunology, Razi Vaccine & Serum Research
Institute, Karaj, Iran
6. PhD
of Microbiology, Razi Vaccine & Serum Research
Institute, Karaj, Iran
7. BSc
of Medical laboratory sciences, Razi Vaccine &
Serum Research Institute, Karaj, Iran
8. MSc
of Microbiology,
Razi Vaccine & Serum Research Institute, Karaj, Iran
9. BSc
of Microbiology, Islamic Azad University, Sanandaj
Branch, Iran
10. MSc
of Microbiology, Islamic Azad University, Karaj, Iran
11. MSc of Microbiology, Baqiyatallah
University of Medical Sciences, Iran
12. MSc of Economy, Islamic Azad University, Firouz
Kouh, Iran
Abstract
Background and Objective:A high level of homogeneity observed within all bacteria in the Mycobacterium
tuberculosis complex makes a property that seriously challenges traditional
biochemical-based identification methods of these pathogens in the laboratory.
The work presented here was conducted to characterize Mycobacterium
tuberculosis complex isolates in Golestan, Northern
Iran.
Material and Methods: Between 2008 and 2010, 42 mycobacterial isolates were collected
from clinical tuberculosis-suspected patients in Golestan
province. The isolates were sub-cultured on fresh Mycobacterium-specific culture
media including glycerinated and pyruvated
Lowenstein-Jensen slopes. The isolates were subsequently subjected to a
Results: All isolates were proved to be M.
tuberculosis. Furthermore, none of the patients were being infected
with any other member of the M. tuberculosis complex or simultaneously
co-infected with two mycobacteria. This fundamental
observation was independently obtained by specific culture media, RV typing and also RD typing.
Conclusion: Considering the fact that cattle
and sheep farming play an important role in the economy of the region, absence
of Mycobacterium bovis in the studied isolates
can be unexpected to some extent. Huard-Warren which is a
simple and cost-effective identification method can be used in both reference
and regional laboratory for differential diagnosis of tuberculosis.
Keywords:
Mycobacterium Tuberculosis Complex, Huard-WarrenMethod, 16SrRNA, Golestan
Province, RD
Typing, RV Typing
Corresponding Author: Tadayon, K.
Email: k.tadayon@rvsri.ac.ir
Received 3 Jan 2012
Revised 10 Jun 2013 Accepted 16 Jul 2013