This paper should be cited as:
Hasannejad Bibalan, M. Javid, N. Samet, M. Shakeri, F. Ghaemi, EA.
Biofilm Formation in Staphylococcus Aureus and its
Relation to Phenotypic and Genotypic Criteria
Hasannejad Bibalan, M. (MSc)1, Javid, N.
(MSc)2, Samet, M. (MSc)3,
Shakeri, F. (MSc)4, Ghaemi,
EA. (PhD)5
1.PhD Student of Bacteriology,
Department of Microbiology, Iran University of Medical
Sciences, Tehran Iran
2.MSc of Microbiology, Department
of Microbiology, Golestan University of Medical
Sciences, Gorgan, Iran
3.MSc of Microbiology, Department
of Microbiology, Golestan University of Medical
Sciences, Gorgan, Iran
4 MSc of Microbiology,
Department of Microbiology, Golestan University of Medical
Sciences, Gorgan, Iran
5.Professor of Microbiology, Department
of Microbiology, Golestan University of Medical
Sciences, Gorgan, Iran
Abstract
Background and Objective:Biofilm is a complex microbial community embedded in a self-produced extracellular
polymeric matrix. We aimed to study the extent of biofilm formation by S. Areas
isolates and its relation to some phenotypic and genotypic criteria.
Material and Methods:One hundred-fifty strains of Staphylococcus
aureus isolated from Gorgan
were studied. Microtiter plate assay method was used for
investigation of biofilm formation.The biofilm formation of strains were recorded and its relation to accessory gene regulator (agr) and antibiotic resistance were assessed by X2
test.
Results:Eighty-four isolates
(56%) were able to form biofilm. The strength of biofilm formation in agr group I was more than that of other groups. The biofilm
formation among S. Areas isolated from the wound and urine (both with 75
%) had the highest capability. Methicillin-resistant
isolates had a greater ability to biofilm formation.
Conclusion:Methicillin resistant isolates had a greater ability to biofilm formation. Given the importance and treatment related problems of
Methicillin-Resistant Staphylococcus Aureus (MRSA) especially Community Acquired-Methicillin-Resistant
Staphylococcus Aureus (CA-MRSA),
it is a necessity to control or remove the biofilm formation alongside
antibiotic treatment.
Keywords: Staphylococcus Aureus, Biofilm, Microtiter Plates Assay, PCR
Corresponding Author: Ghaemi, EA.
Email: eghaemi@yahoo.com
Received:
24 Aug 2013
Revised: 5 Dec 2013
Accepted: 7 Dec 2013