This paper should be cited as: Dadgar, T. Ghaemi, EA. Bahador, N. Imani Fooladi, AA. Kamareie, F.

Detection of Staphylococcus Aureus Enterotoxin Genes A-E

Dadgar, T. (PhD)1*, Ghaemi, EA. (PhD)2, Bahador, N. (PhD)3, Imani Fooladi, AA. (PhD)4, Kamareie, F. (MSc)5

1. PhD of Microbiology, Department of Science and Research, Islamic Azad University, Fars, Iran.

2. Professor of Microbioligy, Infectious Diseases Research Center, Golestan University of Medical Sciences, Gorgan, Iran

3. Assistant Professor of Microbiology, Department of Science and Research, Islamic Azad University, Fars, Iran

4. Associate Professor of Bacteriology, Applied Microbiology Research Center, Baqiyatallah University of Medical Sciences

5. MSc of Microbiology, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran

Abstract

Background and Objective: The main cause of spreading staphylococcal infections among patients is the healthy carriers working in hospitals. With the secretion of different sorts of toxins such as entrotoxin, this bacteria can provide the conditions for attacking on the host. The main objective of this study is identification of the characteristics and differences in the Staphylococcus aureus isolated from healthy carriers and from the patients on the basis of enterotoxin genes (sea-see).

Material and Methods: One hundred and twenty of the patients and 80 of healthy carriers worked in health centers of Gorgan, north of Iran, were investigated for S. aureus isolate. The isolates were evaluated by PCR for Enterotoxin Genes A-E (SEA to SEE).

Results: Enterotoxin genes (SEA to SEE) was found in 87.5% of the total isolates and the most frequent one was enterotoxin gene sea (N= 124). The prevalence of these isolates in healthy carriers was significantly higher than those of the patients.

Conclusion: Based on the results, the high percentage of S. aureus isolated from clinical samples contains enterotoxin genes. Therefore, Human as the source and carrier of S. aureus is paramount importance, which is due to significant relationship between being toxigenic strains and the source of isolation.

Key words: Staphylococcus Aureus; Enterotoxin; Patient; Carrier

Corresponding Author: Dadgar, T.

Email: dadgar_teena@yahoo.com

Received 29 Nov 2012 Revised 29 Jan 2013 Accepted 30 Jan 2013