This paper should be cited as: Movahedian, A. Puramini, N. Bordbar-Bonab, S. Fattahi, E. Mirza-Aghazadeh, A. Bahrami, A. Fattahi, SH. Dolatkhah, H.
Glycated Hemoglobin, Gastric Juice Nitric Oxide and Oxidative Stress in Diabetic Patients Infected by Helicobacter pylori
Movahedian, A. (PhD)1 Puramini, N. (BSc)2 Bordbar-Bonab, S. (BSc)3 Fattahi, E. (MD)4 Mirza-Aghazadeh, A. (PhD)5 Bahrami, A. (MD)6 Dolatkhah, H. (MSc)7
1.Professor of Clinical Biochemistry, Pharmaceutical Sciences Research Centre, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran
2. BSc of Nursing, Sina Hospital Educational Center, Tabriz University of Medical Sciences, Tabriz, Iran
3. BSc of Genetic, Faculty of Sciences, Islamic Azad University, Ahar Branch, Iran
4. Professor of Liver and Gastrointestinal Diseases, Tabriz Gastrointestinal and Liver Disease Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
5. Assistant Professor of Statistics, Dept. of Basic Sciences, Faculty of Para Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
6. Professor of Endocrinology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
7. PhD Student of Clinical Biochemistry, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran
Abstract
Background and Objective: Recently, diabetes mellitus has been known as one of the main cause of upper gastrointestinal symptoms. Since a high prevalence of H. pylori in diabetic patients has been reported, we aimed to evaluate the level of gastric juice Nitric Oxide (NO°), Oxidative Stress and Glycated Hemoglobin.
Material and Methods: In case group, the participants were 60 diabetic patients infected with H. pylori, and in control groups 60 diabetic patients without H. pylori and 60 healthy individuals. The level of NO° in gastric juice was measured calorimetrically and the activity of superoxide dismutase (SOD) and glutathione peroxidase (GPX) in gastric biopsy was determined using standard methods. The percentage of Glycated Hemoglobin (HbA1C) was measured by ion exchange chromatography.
Results: In case group compared to controls, significantly increased level of blood HbA1C, nitric oxide in gastric juice, activity of SOD and GPX in the gastric mucosa were observed (p<0.0001).
Conclusion: A significant increase of glycated hemoglobin in diabetic patients with H. pylori and high activity of antioxidant enzymes in the case group may indicate a high production of reactive oxygen species and the presence of oxidative stress in these patients.
Key Words: Diabetes Mellitus, H. pylori Infection, Glycated Hb, Nitric Oxide, Oxidative Stress
Corresponding Author: Dolatkhah, H.
Email: dolatkhahh@gmail.com
Received: 31
Aug 2013
Revised: 9 May 2014
Accepted: 10 May 2014