Volume 9, Issue 3 (Jul,Aug2015[PERSIAN] 2015)                   mljgoums 2015, 9(3): 40-46 | Back to browse issues page

XML Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Izadpanah, MR, Asadpour, L. Molecular Identification and Antibacterial Drug Resistance Pattern of Staphylococcus Aureus Isolated in Rasht, Iran. mljgoums 2015; 9 (3) :40-46
URL: http://mlj.goums.ac.ir/article-1-738-en.html
1- Islamic Azad University, Guilan Science and Research Branch, Rasht, Iran
2- Department of Veterinary Science, Rasht Branch, Islamic Azad University, Rasht, Iran
Abstract:   (16336 Views)

Abstract

Background and Objective: Staphylococcus aureus is an important opportunistic pathogen causing a wide range of infections in human .Most clinical isolates of S.aureus are resistant to a number of antibiotics. For appropriate antimicrobial therapy, this study was conducted to determine antibacterial drug resistance patterns of S.aureus isolates obtained from different clinical samples in Rasht.

Material and Methods:  the clinical isolates of S.aureus were collected from different clinical laboratories in Rasht. Thirty coagulase positive S.aureus strains were identified using biochemical tests and amplification of 23SrRNA and coa genes by polymerase chain reaction.  Finally, the resistance pattern of the isolates to 16 selected antimicrobial agents was evaluated by disk diffusion method.

Results:  the S.aureus isolates (75%) were resistant to methicillin and all of them were multidrug resistance. The isolates were high resistance to ampicillin (73%), amoxicillin (60%), cloxacillin (53%) and low resistance to vancomycin (7%) and gentamicin (10%).

Conclusion: given the high prevalence of methicillin resistant, multi drug resistant and presence of vancomycin resistant S.aureus isolates in Rasht, continuously monitoring of drug resistance pattern of S.aureus isolates is recommended for having appropriate therapeutic regime.

Keywords: Staphylococcus Aureus, Coagulase, Drug Resistance, PCR

Research Article: Original Paper |
Received: 2015/10/11 | Accepted: 2015/10/11 | Published: 2015/10/11 | ePublished: 2015/10/11

Add your comments about this article : Your username or Email:
CAPTCHA

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2007 All Rights Reserved | Medical Laboratory Journal

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.