Volume 16, Issue 5 (Sep-Oct 2022)                   mljgoums 2022, 16(5): 1-2 | Back to browse issues page


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1- Laboratory Science Research Center, Faculty of Paramedicine, Golestan University of Medical Sciences, Gorgan, Iran , kalavi@goums.ac.ir
2- Department of Medical Genetics, Shiraz University of Medical Sciences, Shiraz, Iran, and Department of Psychiatry and Behavioral Sciences, Miami, USA
3- Laboratory Science Research Center, Faculty of Paramedicine, Golestan University of Medical Sciences, Gorgan, Iran
4- DarouPakhsh Distribution Vet. Company, Iran
Abstract:   (1164 Views)
Dear Editor,
There are several reports about the modulatory effects of intracellular pathogens, such as Leishmania spp., to help survival and replication inside host cells. They alter host cells' defense and offence mechanisms, and in case of leishmaniasis, the pathogen creates a hostile environment inside macrophages. We performed an RNA sequencing analysis of transcriptome changes in Leishmania major-infected human macrophages at four hour post infection. In this study, we investigated gene expression pattern of the infected macrophages against microbead (4.16 µm) polystyrene particles phagocytized and non-polarized macrophages as controls. Monocytes with high purity were isolated from healthy donors by magnetic-activated cell sorting and then differentiated into macrophages after 6-9 days of incubation at 37 ˚C.
In this study, we focused on some key interaction events between host cell and the pathogen; so some feature steps resulting from dictation strategies of the pathogen are presented below.
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Research Article: Letter to Editor | Subject: Human Genetics
Received: 2020/10/5 | Accepted: 2020/12/15 | Published: 2022/07/16 | ePublished: 2022/07/16

References
1. de Morais CG, Castro Lima AK, Terra R, dos Santos RF, Da-Silva SA, Dutra PM. The Dialogue of the Host-Parasite Relationship: Leishmania spp. and Trypanosoma cruzi Infection. Biomed Res Int. 2015;2015:324915. [View at Publisher] [DOI:10.1155/2015/324915] [PubMed] [Google Scholar]
2. Adewale Fadaka, Basiru Ajiboye, Oluwafemi Ojo, Olusola Adewale, Israel Olayide, Rosemary Emuowhochere: Biology of glucose metabolization in cancer cells; journal of oncological sciences. 2017; 3(6); 45-51. [View at Publisher] [DOI:10.1016/j.jons.2017.06.002] [Google Scholar]
3. Cekic C, Sag D, Day YJ, Linden J. Extracellular adenosine regulates naive T cell development and peripheral maintenance. J Exp Med. 2013; 210(12): 2693-706. [View at Publisher] [DOI:10.1084/jem.20130249] [PubMed] [Google Scholar]
4. Campesato LF, Budhu S, Tchaicha J, Weng CH, Gigoux M, Cohen IJ, et al. Blockade of the AHR restricts a Treg-macrophage suppressive axis induced by L-Kynurenine. Nat Commun. 2020; 11(1): 4011. [View at Publisher] [DOI:10.1038/s41467-020-17750-z] [PubMed] [Google Scholar]

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