Volume 14, Issue 1 (Jan-Feb 2020)
mljgoums 2020, 14(1): 29-35 |
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1- Assoociated Professor Microbiologist, Faculty of Veterinary Medicine, Shiraz University, Shiraz, Iran , mtabatabaei2003@yahoo.co.uk
2- M.Sc. student in bacteriology, Faculty of Veterinary Medicine, Shiraz University, Shiraz, Iran
3- Professor, Pathologist, Shiraz Transplant Research Center, Shiraz University of Medical SciencesShiraz, Iran
4- Surgeon, Trauma Research Center, Shiraz University of Medical Sciences, Shiraz, Iran,
2- M.Sc. student in bacteriology, Faculty of Veterinary Medicine, Shiraz University, Shiraz, Iran
3- Professor, Pathologist, Shiraz Transplant Research Center, Shiraz University of Medical SciencesShiraz, Iran
4- Surgeon, Trauma Research Center, Shiraz University of Medical Sciences, Shiraz, Iran,
Abstract: (4032 Views)
ABSTRACT
Background and Objective: Bilophlia spp. are gram-negative, pleomorphic rod, obligate anaerobe, oxidase-negative, catalase-positive and non-motile bacteria. B. wadsworthia is type species of genus Bilophila with the additional characteristic of urea hydrolysis. B. wadsworthia can be found in a variety of anaerobe infections, particularly appendicitis and intra-abdominal infection that are considered as important opportunistic pathogens.
Methods: This study was designed to identify Bilophila spp. in clinical specimens by culture and PCR. We examined 91 DNA samples extracted from infected appendix tissues with specific primers.
Results: Data showed that Bilophila spp. DNA existence in 53.85% (n=49) provided appendiceal tissue.
Conclusion: The pathological and molecular examination of infected appendiceal tissues revealed that B. wadsworthia is able to act as the primary cause of significant lesions in the appendicle tissues.
Key words: Bilophila spp., Appendectomy, Appendicle specimens, PCR, Nucleotide sequencing
Background and Objective: Bilophlia spp. are gram-negative, pleomorphic rod, obligate anaerobe, oxidase-negative, catalase-positive and non-motile bacteria. B. wadsworthia is type species of genus Bilophila with the additional characteristic of urea hydrolysis. B. wadsworthia can be found in a variety of anaerobe infections, particularly appendicitis and intra-abdominal infection that are considered as important opportunistic pathogens.
Methods: This study was designed to identify Bilophila spp. in clinical specimens by culture and PCR. We examined 91 DNA samples extracted from infected appendix tissues with specific primers.
Results: Data showed that Bilophila spp. DNA existence in 53.85% (n=49) provided appendiceal tissue.
Conclusion: The pathological and molecular examination of infected appendiceal tissues revealed that B. wadsworthia is able to act as the primary cause of significant lesions in the appendicle tissues.
Key words: Bilophila spp., Appendectomy, Appendicle specimens, PCR, Nucleotide sequencing
Research Article: Original Paper |
Subject:
Microbiology
Received: 2018/07/15 | Accepted: 2019/09/28 | Published: 2019/12/30 | ePublished: 2019/12/30
Received: 2018/07/15 | Accepted: 2019/09/28 | Published: 2019/12/30 | ePublished: 2019/12/30
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