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Abouhosseini Tabari M, Youssefi M R, Hosseini Farash B R, Ebrahimi M A, Nabavi Mousavi N, Moghaddas E. Anti-Leishmanial Activity of Pelargonium roseum Essential Oil on Growth of Leishmania infantum Promastigotes . mljgoums 2017; 11 (5) :36-40
URL: http://mlj.goums.ac.ir/article-1-1009-en.html
URL: http://mlj.goums.ac.ir/article-1-1009-en.html
Mohaddeseh Abouhosseini Tabari1 
, Mohammad Reza Youssefi2 , Bibi Razieh Hosseini Farash3 , Mohammad Amin Ebrahimi4 , Niki Nabavi Mousavi5 , Elham Moghaddas 6
, Mohammad Reza Youssefi2 , Bibi Razieh Hosseini Farash3 , Mohammad Amin Ebrahimi4 , Niki Nabavi Mousavi5 , Elham Moghaddas 6
1- Faculty of Veterinary Medicine, Amol University of Special ModernTechnologies, Amol, Iran
2- Department of Veterinary Parasitology, Babol-Branch, Islamic Azad University, Babol, Iran
3- Department of Parasitology and Mycology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
4- Young Researcher and Elite Club, Babol -Branch,Islamic Azad University, Babol-Branch, Babol, Iran
5- Young Researcher and Elite Club, Babol- Branch, Islamic Azad University, Babol-Branch, Babol, Iran
6- Department of Parasitology and Mycology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran , moghaddase@mums.ac.ir
2- Department of Veterinary Parasitology, Babol-Branch, Islamic Azad University, Babol, Iran
3- Department of Parasitology and Mycology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
4- Young Researcher and Elite Club, Babol -Branch,Islamic Azad University, Babol-Branch, Babol, Iran
5- Young Researcher and Elite Club, Babol- Branch, Islamic Azad University, Babol-Branch, Babol, Iran
6- Department of Parasitology and Mycology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran , moghaddase@mums.ac.ir
Abstract: (11141 Views)
ABSTRACT
Background and Objectives: Due to adverse effects of pentavalent antimonial treatment of visceral leishmaniasis using herbal medicines has received a lot of attention. In this study, we evaluated inhibitory activity of Pelargonium roseum essential oil on growth of Leishmania infantum promastigotes, and compared it with that of Glucantime.
Methods: Inhibitory effects of different concentrations (1, 2.5, 5, 10, 25, 50 and 100 µL/ml) of P. roseum essential oil on the growth of L. infantum promastigotes were studied by MTT assay after 24 and 48 hours. All the data were analyzed by one-way analysis of variance. The effect of the essential oil was later compared with that of standard dose of Glucantime.
Results: After 24 hours, different concentrations of the essential oil had no significant inhibitory effect on promastigotes of L. infantum. After 48 hours, all concentrations except for 1µL/ml showed significant anti-leishmanial activity. In addition, the lowest concentration that had significant inhibitory effect on L. infantum promastigote was 5 µL/ml.
Conclusion: The essential oil of P. roseum has favorable inhibitory activity on the growth of L. infantum promastigotes after 48 hours. This indicates the therapeutic potential of this plant for treatment of leishmaniasis.
Keywords: Leishmania infantum, Pelargonium, Promastigote, Meglumine antimoniate.
Background and Objectives: Due to adverse effects of pentavalent antimonial treatment of visceral leishmaniasis using herbal medicines has received a lot of attention. In this study, we evaluated inhibitory activity of Pelargonium roseum essential oil on growth of Leishmania infantum promastigotes, and compared it with that of Glucantime.
Methods: Inhibitory effects of different concentrations (1, 2.5, 5, 10, 25, 50 and 100 µL/ml) of P. roseum essential oil on the growth of L. infantum promastigotes were studied by MTT assay after 24 and 48 hours. All the data were analyzed by one-way analysis of variance. The effect of the essential oil was later compared with that of standard dose of Glucantime.
Results: After 24 hours, different concentrations of the essential oil had no significant inhibitory effect on promastigotes of L. infantum. After 48 hours, all concentrations except for 1µL/ml showed significant anti-leishmanial activity. In addition, the lowest concentration that had significant inhibitory effect on L. infantum promastigote was 5 µL/ml.
Conclusion: The essential oil of P. roseum has favorable inhibitory activity on the growth of L. infantum promastigotes after 48 hours. This indicates the therapeutic potential of this plant for treatment of leishmaniasis.
Keywords: Leishmania infantum, Pelargonium, Promastigote, Meglumine antimoniate.
Research Article: Original Paper |
Received: 2017/11/15 | Accepted: 2017/11/15 | Published: 2017/11/15 | ePublished: 2017/11/15
Received: 2017/11/15 | Accepted: 2017/11/15 | Published: 2017/11/15 | ePublished: 2017/11/15
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