en بیوشیمی Biochemistry Research Article Research Article <div style="text-align: justify;"><span style="font-size:12px;"><span style="font-family:Times New Roman;"><b>Background:</b> DNA glycation, a process where Glc non-enzymatically binds to DNA, is implicated in various detrimental effects, including strand breaks, mutations, and altered gene expression. This damage is considered a significant contributor to the pathogenesis of diabetes mellitus and its associated complications. Consequently, there has been increasing interest in identifying antiglycation agents as a strategy for preventing and mitigating these complications. Prior research has indicated that extracts from <i>Tamarix aphylla</i> (<i>T. aphylla</i>) leaves possess antidiabetic properties. Therefore, this study aimed to investigate, for the first time, the impact of <i>T. aphylla</i> extract on Glc-mediated DNA glycation.<br> <b>Methods:</b> DNA samples were incubated with Glc over a four-week period. Subsequently, the modulatory effects of <i>T. aphylla</i> on Glc-induced DNA structural alterations were investigated employing a range of analytical techniques. These methodologies encompassed ultraviolet-visible (UV-Vis) spectroscopy, fluorescence spectroscopy, circular dichroism (CD) spectroscopy, and agarose gel electrophoresis.<br> <b>Results:</b> The results obtained from UV&ndash;Vis and fluorescence spectroscopy demonstrated that <i>T. aphylla</i> extract led to a reduction in the formation of DNA-advanced glycation end products (AGEs). Furthermore, CD spectroscopy and agarose gel electrophoresis analyses indicated that the structural alterations of glycated DNA were diminished in the presence of <i>T. aphylla</i> extract.<br> <b>Conclusion</b>: Based on the evidence presented, <i>T. aphylla</i> demonstrates protective properties against DNA glycation. Consequently, pending further rigorous investigation, it may represent a potentially valuable therapeutic agent for mitigating the detrimental consequences of glycation, particularly in environments characterized by elevated Glc concentrations and hyperglycemic states.</span></span></div> DNA, Glucose, Glycation, Tamarix aphylla 34 37 http://mlj.goums.ac.ir/browse.php?a_code=A-10-1798-1&slc_lang=en&sid=1 Parisa Hasanein parisa.hasanein@gmail.com 100319475328460030525 100319475328460030525 Yes Department of Biology, School of Basic Sciences, University of Zabol, Zabol, Iran Fahime Javadi Hedaiat Abad fahimejavadihedaiat@gmail.com 100319475328460030526 100319475328460030526 No Department of Biology, School of Basic Sciences, University of Zabol, Zabol, Iran Mousa Bohlooli bohlooli@alumni.ut.ac.ir 100319475328460030527 100319475328460030527 No Department of Biology, School of Basic Sciences, University of Zabol, Zabol, Iran; Department of Cell and Molecular Sciences, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran Mostafa Khajeh mahsamaleki89@gmail.com 100319475328460030528 100319475328460030528 No Department of Chemistry, School of Basic Sciences, University of Zabol, Zabol, Iran Sedigheh Esmaielzadeh Bahabadi n.poormolaie@gmail.com 100319475328460030529 100319475328460030529 No Department of Biology, School of Basic Sciences, University of Zabol, Zabol, Iran Neda Poormolaei npoormolaie@gmail.com 100319475328460030530 100319475328460030530 No Department of Biology, School of Basic Sciences, University of Zabol, Zabol, Iran