Medical Laboratory Journal
Medical Laboratory Journal
mljgoums
Medical Sciences
http://mlj.goums.ac.ir
1
admin
2538-4449
10.52547/mlj
en
jalali
1396
4
1
gregorian
2017
7
1
11
4
online
1
fulltext
fa
Prevalence of Active Cytomegalovirus Infection in Hemodialysis Patients
تحقيقي
Original Paper
<div><strong>ABSTRACT</strong><br>
<strong> Background and Objectives</strong><strong><span dir="RTL">:</span></strong> Human cytomegalovirus (HCMV) is the most common viral cause of morbidity and mortality in immunocompromised patients<strong>. </strong>The aim of this study was to evaluate the frequency of active CMV infection in hemodialysis patients in Gorgan, Iran.<br>
<strong> Methods: </strong>Plasma samples were obtained from 149 hemodialysis patients at Hemodialysis Unit of Panje-Azar Medical Centre in Gorgan, Iran. Presence of <a name="OLE_LINK194"></a><a name="OLE_LINK195"></a><a name="OLE_LINK201"></a><a name="OLE_LINK200">CMV-DNA </a>in plasma samples was evaluated by polymerase chain reaction (<a name="OLE_LINK94"></a><a name="OLE_LINK91">PCR</a>) using specific primers for highly conserved regions of major capsid protein gene of HCMV. In addition, level of CMV-IgM antibody was measured by serological testing. Demographic information and past medical history of patients were also recorded. Data was analyzed by SPSS software (version 18).<br>
<strong> Results:</strong> Total prevalence of CMV infection was 6.7% (10/149) among the patients receiving <a name="OLE_LINK84">hemodialysis</a>. CMV-DNA and anti-CMV IgM antibody were detected in 2.68% and 4.69%, of the samples, respectively. One case was found positive for both CMV-DNA and anti-CMV IgM antibody. CMV infection did not have any correlation with gender, age, ethnicity, duration of hemodialysis, and history of blood transfusion.<br>
<strong> Conclusion: </strong>A notable proportion of hemodialysis patients in Gorgan have active CMV infection. Accurate detection of these individuals is important for preventing infection spread, especially in immunocompromised individuals. Simultaneous diagnosis of CMV infection using serological testing and PCR assay could help reduce the risk of infection spread.<br>
<strong> Keywords: </strong><a name="OLE_LINK104"></a><a name="OLE_LINK103">HCMV</a>, Hemodialysis, PCR, Iran.</div>
HCMV, Hemodialysis, PCR, Iran.
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http://mlj.goums.ac.ir/browse.php?a_code=A-10-1-470&slc_lang=fa&sid=1
Mishar
Kelishadi
100319475328460013934
100319475328460013934
Yes
Student Research Committee, Department of Microbiology, Golestan University of Medical Sciences, Gorgan, Iran
Mohammad
Mojerloo
100319475328460013935
100319475328460013935
No
Department of Hemodialysis, 5th Azar Hospital, Gorga, Iran
Pezhman
Hashemi
100319475328460013936
100319475328460013936
No
Student Research Committee, Department of Microbiology, Golestan University of Medical Sciences, Gorgan, Iran
Sobhan
Samadi
100319475328460013937
100319475328460013937
No
Student Research Committee, Department of Microbiology, Golestan University of Medical Sciences, Gorgan, Iran
Alijan
Tabarraei
100319475328460013938
100319475328460013938
No
Infectious Diseases Research Center, Department of Microbiology، Golestan University of Medical Sciences, Gorgan, Iran