Medical Laboratory Journal
Medical Laboratory Journal
mljgoums
Medical Sciences
http://mlj.goums.ac.ir
1
admin
2538-4449
10.52547/mlj
en
jalali
1395
8
1
gregorian
2016
11
1
10
6
online
1
fulltext
fa
In vitro Susceptibility Study of Salmonella typhimurium Mutant to Stress Factors
تحقيقي
Original Paper
<div>
<p><strong>ABSTRACT</strong></p>
<p><strong> Background and Objective:</strong> In this study, we compared the susceptibility of <em>Salmonella typhimurium</em> <em>phoP</em> mutant and its parent to stress conditions that the pathogen may encounter in a host.</p>
<p><strong> Methods: </strong>For this purpose, we used the <em>phoP</em> deletion mutant constructed in our previous study. In order to test the in vitro susceptibility of the mutant to stress factors, the effect of acidic pH, heat, bile salts and polymyxin on growth of the mutant was examined. Then, minimum inhibitory concentration and minimum bactericidal concentration of bile salts and polymyxin were determined. <em>Salmonella typhimurium</em> 14028 was used as the parent strain.</p>
<p><strong> Results:</strong> The mutant was highly susceptible to bile salts and polymyxin in comparison with the parent strain, but no difference was observed in their susceptibility to acid and heat.</p>
<p><strong> Conclusion: </strong>This study confirms the role of the <em>phoP</em> in resistance of <em>Salmonella</em> to polymyxin and bile salts. Mutation in the <em>phoP</em> leads to susceptibility of the mutant to bile and cationic antimicrobial peptides.</p>
<p><strong> Keywords:</strong> In vitro, Mutant, <em>Salmonella typhimurium</em>, <a href="https://www.ncbi.nlm.nih.gov/mesh/68004198">Disease Susceptibility</a>.</p>
</div>
In vitro, Mutant, Salmonella typhimurium, Disease Susceptibility.
28
31
http://mlj.goums.ac.ir/browse.php?a_code=A-10-1-436&slc_lang=fa&sid=1
Ania
Ahani Azari
ania_783@yahoo.com
100319475328460012466
100319475328460012466
Yes
Department of Microbiology, Gorgan Branch
Taghi
Zahraei Salehi
100319475328460012467
100319475328460012467
No
Department of Microbiology, Faculty of Veterinary Medicine
Bahar
Nayeri Fasaei
100319475328460012468
100319475328460012468
No
Department of Microbiology, Faculty of Veterinary Medicine