Medical Laboratory Journal
Medical Laboratory Journal
mljgoums
Medical Sciences
http://mlj.goums.ac.ir
1
admin
2538-4449
10.52547/mlj
en
jalali
1393
10
1
gregorian
2015
1
1
8
5
online
1
fulltext
fa
سروتایپینگ سالمونلا در خامه های غیر پاستوریزه عرضه شده در همدان و تعیین الگوی مقاومت آنتی بیوتیکی آنها
Serotyping of Salmonella in Unpasteurized Cream Samples and Their Antibiotic Resistance Pattern
تحقيقي
Original Paper
Abstract Background and Objective: Cream, a rich dairy product, with a neutral PH and low preservation time is a suitable medium for microbial growth. Salmonella is one of the most important pathogens in causing food poisoning and human gastroenteritis. This study aimed at investigating the quality of traditional cream for the bacterial contamination. Material and Methods: In this cross-sectional study, 196 non-pasteurized cream samples were collected from 5 regions of Hamedan, Iran. After dilution in phosphate buffer and serial dilution preparation, Salmonella was transferred to Rappaport-Vassiliadis (RV) enrichment medium according to CDC guidelines. After 24 h incubation at 37 ° C, a loop was inoculated in MacConkey and Hektoen Enteric (HE) Agar. The suspected colony phenotype was examined and their identification confirmed by API-20 E. Results: The samples (29%) were contaminated with at least one kind of bacteria, Salmonella Spp (4.59%) and Yersinia Spp (2.55%). The other bacteria like Escherichia, Enterobacter, Klebsiella, and Citrobacter were also isolated. Nine samples were contaminated with two kinds of bacteria. Conclusion: The presence of bacteria such as Salmonella and Yersinia in unpasteurized cream indicates that more quality control needs to be applied to the traditional crème produced in the city by health control office of food products. Keywords: Cream, Salmonella SPP, Coliform, Yersinia Enterocolitica, Hamedan
Cream, Salmonella SPP, Coliform, Yersinia Enterocolitica, Hamedan
22
27
http://mlj.goums.ac.ir/browse.php?a_code=A-10-1-265&slc_lang=fa&sid=1
Ghanadan, M
مژگان قنادان
100319475328460012651
100319475328460012651
No
Islamic Azad University, Science and Research Branch, Tehran, Iran
دانشگاه آزاد اسلامی واحد علوم و تحقیقات اراک
Akbari, N
ندا اکبری
100319475328460012652
100319475328460012652
No
Islamic Azad University, , Science and Research Branch, Tehran, Iran.
دانشگاه آزاد اسلامی واحد تهران
Soltan Dallal, MM
محمد مهدی سلطان دلال
soltanirad34@yahoo.co
100319475328460012653
100319475328460012653
Yes
Deptartment of Pathobiology, School of Public Health Tehran University of Medical Sciences, Tehran, Iran
دانشگاه علوم پزشکی تهران