Medical Laboratory Journal
Medical Laboratory Journal
mljgoums
Medical Sciences
http://mlj.goums.ac.ir
1
admin
2538-4449
10.52547/mlj
en
jalali
1391
1
1
gregorian
2012
4
1
6
1
online
1
fulltext
fa
فراوانی اشریشیا کلی های مولد آنزیم بتالاکتاماز وسیع الطیف در گرگان
Extended spectrum beta lactamase producing E.coli isolated from Gorgan, North of Iran
تحقيقي
Original Paper
Abstract Background and objectives: The increase of ESBL producing E.coli can create a tremendous difficult y for the health system. These isolates leads to rapid transmission of causative genes to other clinically important bacteria and synchronously increased resistant to other antibiotics. This study was carried out to determine the prevalence of this isolate and related genes in Gorgan, North of Iran. Material and Methods: This study was conducted on 218 isolated E.coli from urinary tract infection of outpatients referring to six medical laboratories in Gorgan, during 2010-11. The resistance to Cefotaxim (Mast Co.) was assessed by Kirby-Bauer disk diffusion method. The confirmatory test for detection of resistant isolates was carried out by double disk method at the presence of Cefotaxim and clavulanic acid. The presence of β lactamase gene of blatem, blactx and blashv in ESBL was assessed by PCR method. Results: of 218, 70 isolates (32.1%) are resistant to Cefotaxim. Sixty-two (88.6%) of them are confirmed as ESBL producing E.coli. β lactamase genes of blactx, blatem and blashv can be seen in 28(45.2%), 26(41.9%) and 6(9.7%) isolates, respectively. Conclusion: the prevalence of ESBL producing E.coli in Gorgan is in the range of country average and blaCTX-M gene is the most common gene. Key words: E.coli, ESBL, bla gene, UTI
E.coli, ESBL, bla gene, UTI
51
58
http://mlj.goums.ac.ir/browse.php?a_code=A-10-1-111&slc_lang=fa&sid=1
Babaii Kochaksaraii M
مایا بابائی کوچکسرائی
mayasayeh@gmail.com
100319475328460013487
100319475328460013487
Yes
Master of Microbiology
NasrolahiOmran A
آیت الله نصرالهی
100319475328460013488
100319475328460013488
No
Assisatnat Professor, Islamic
Javid N
نائمه جاوید
100319475328460013489
100319475328460013489
No
Master of Microbiology
Shakeri F
فاطمه شاکری
100319475328460013490
100319475328460013490
No
Master of Microbiology
Yazdi M
مهسا یزدی
100319475328460013491
100319475328460013491
No
Master of Microbiology
Ghaemi E A
عزت الله قائمی
100319475328460013492
100319475328460013492
No
Associate Professor, Infectious Disease Research Center
مرکز تحقیقات عفونی