Medical Laboratory Journal
Medical Laboratory Journal
mljgoums
Medical Sciences
http://mlj.goums.ac.ir
1
admin
2538-4449
10.52547/mlj
en
jalali
1399
12
1
gregorian
2021
3
1
15
2
online
1
fulltext
en
Effect of Glucosamine Sulfate Alone and Combined with Moderate Intensity Exercise on Serum Levels of CS 846 Epitope and Cartilage Oligomeric Matrix Protein in a Rat Model of Osteoarthritis
فیزیولوژی ورزشی
Sport Physiology
تحقيقي
Original Paper
<strong>Background and objectives:</strong> Osteoarthritis is the result of a defect in synovial membrane-covered joint tissues. The purpose of this study was to investigate effects of glucosamine sulfate alone and combined with moderate intensity exercise on serum levels of CS 846 epitope and cartilage oligomeric matrix protein (COMP) in a rat model of osteoarthritis.<br>
<strong>Methods:</strong> In this study, after inducing osteoarthritis in 42 male Wistar rats (weighting 250±300 g, aged 8 to 12 weeks), the rats were randomly divided into five groups: control-healthy, control-patient, patient-exercise, patient-glucosamine and patient-glucosamine-exercise. The training program consisted of 30 minutes of running on a non-slip treadmill at speed of 16 m/min in the first week with progressive overload principle reaching 50 minutes by the eighth week. The glucosamine groups received oral glucosamine sulfate (250 mg/kg/day) once a day for eight consecutive weeks. The serum levels of CS 846 epitope and COMP were measured using commercial ELISA kits. Data were analyzed using one-way ANOVA and Tukey's post hoc test. All statistical analyses were performed in GraghPad prism 8 and at significance level of 0.05.<br>
<strong>Results:</strong> Combined exercise and glucosamine supplementation caused a significant decrease in the COMP and CS846<a> levels.</a> This decrease was more profound compared to that of glucosamine and exercise alone.<br>
<strong>Conclusion: </strong>Overall, the findings of the present study showed that osteoarthritis increases serum COMP and CS 846 levels. In addition, glucosamine supplementation combined with exercise can significantly improve knee osteoarthritis in rats.
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Cartilage oligomeric matrix protein,epitopes,Exercise,Glucosamine,Osteoarthritis,
35
41
http://mlj.goums.ac.ir/browse.php?a_code=A-10-849-1&slc_lang=en&sid=1
babak
Biniaz
biniazbabak@gmail.com
100319475328460019131
100319475328460019131
No
Department of Sport Physiology, Sari Branch, Islamic Azad University, Sari, Iran
hajar
abaszadeh
h.abaszade61@gmail.com
100319475328460019132
100319475328460019132
Yes
Department of Sport Physiology, Sari Branch, Islamic Azad University, Sari, Iran
parvin
farzanegi
parvin.farzanegi@gmail.com
100319475328460019133
100319475328460019133
No
Department of Sport Physiology, Sari Branch, Islamic Azad University, Sari, Iran