Medical Laboratory Journal
Medical Laboratory Journal
mljgoums
Medical Sciences
http://mlj.goums.ac.ir
1
admin
2538-4449
10.52547/mlj
en
jalali
1396
6
1
gregorian
2017
9
1
11
5
online
1
fulltext
fa
Frequency of Adherence Genes cna, fnbA and fnbB in Staphylococcus aureus Isolates from Traditional Cheese
تحقيقي
Original Paper
<div><strong>ABSTRACT</strong><br>
<strong> Background and Objectives:</strong> Several virulence factors are involved in the pathogenesis of <em>Staphylococcus aureus</em>. Surface proteins such as collagen binding proteins (<em>Cna</em>) and fibronectin-binding proteins (FnBP) are important factors in adhesion and invasion of <em>S. aureus</em>. The aim of this study was to evaluate the frequency of adherence genes <em>cna</em>, <em>fnbA</em> and <em>fnbB</em> <em>S. aureus</em> isolates from traditional cheese.<br>
<strong> Methods:</strong> All 22 isolates tested were identified as <em>S. aureus</em>. The isolates were tested for the presence of adherence genes <em>cna</em>, <em>fnbA</em> and <em>fnbB</em> using specific primers in polymerase chain reaction assay. <br>
<strong> Results:</strong> Six isolates (27.27%) were positive for the <em>can</em> gene. Of the 22 isolates studied, one isolate was positive for <em>fnbA</em> and one was positive for the <em>fnbB</em>. Co-presence of the genes examined was not observed in any of the isolates.<br>
<strong> Conclusion:</strong> The results indicate the weak biofilm formation ability of the <em>S. aureus</em> isolates from traditional cheese.<br>
<strong> Keywords</strong>: <em>Staphylococcus aureus</em>, Biofilm, Genes, Cheese. </div>
Staphylococcus aureus, Biofilm, Genes, Cheese.
11
15
http://mlj.goums.ac.ir/browse.php?a_code=A-10-1-481&slc_lang=fa&sid=1
Bahareh
Charmi far
100319475328460013914
100319475328460013914
No
Department of Microbiology, Urmia Branch, Islamic Azad University, Urmia, Iran
Saman
Mahdavi
S.mahdavi@iau-maragheh.ac.ir
100319475328460013915
100319475328460013915
Yes
Department of Microbiology, Maragheh Branch, Islamic Azad University, Maragheh, Iran