@ARTICLE{Tabatabaei, author = {Tabatabaei, Mohammad and Dehvari, Aslam and Geramizadeh, Bita and Niakan, Mohammad Hadi and }, title = {Probable Role of Bilophila wadsworthia in Appendicle Infection}, volume = {14}, number = {1}, abstract ={ABSTRACT Background and Objective: Bilophlia spp. are gram-negative, pleomorphic rod, obligate anaerobe, oxidase-negative, catalase-positive and non-motile bacteria. B. wadsworthia is type species of genus Bilophila with the additional characteristic of urea hydrolysis. B. wadsworthia can be found in a variety of anaerobe infections, particularly appendicitis and intra-abdominal infection that are considered as important opportunistic pathogens. Methods: This study was designed to identify Bilophila spp. in clinical specimens by culture and PCR. We examined 91 DNA samples extracted from infected appendix tissues with specific primers. Results: Data showed that Bilophila spp. DNA existence in 53.85% (n=49) provided appendiceal tissue. Conclusion: The pathological and molecular examination of infected appendiceal tissues revealed that B. wadsworthia is able to act as the primary cause of significant lesions in the appendicle tissues. Key words: Bilophila spp., Appendectomy, Appendicle specimens, PCR, Nucleotide sequencing }, URL = {http://mlj.goums.ac.ir/article-1-1105-en.html}, eprint = {http://mlj.goums.ac.ir/article-1-1105-en.pdf}, journal = {Medical Laboratory Journal}, doi = {10.29252/mlj.14.1.29}, year = {2020} }