This paper should be cited as: Daie Ghazvini, R. Khosravi, A. Ghiasian, SA.

Identification of Electrophoretic Protein Pattern of Fusaium Verticillioides Isolates from Maize by SDS-PAGE

Daie Ghazvini, R. (PhD)1, Khosravi, A. (PhD)2, Ghiasian, SA. (PhD)3

1. Assistant Professor of Medical Mycology, Department of Parasitology and Mycology School of Public Health Tehran University of Medical Sciences, Tehran, Iran

2. Professor of Medical Mycology, Mycology Research Centre, Faculty of Veterinary Medicine, Tehran University, Tehran, Iran

3. Associate Professor of Medical Mycology, Department of Parasitology and Mycology, Faculty of Medicine, Hamedan University of Medical Sciences, Hamedan, Iran

Abstract

Background and Objective: Some of them possess high allergenic components and some produce the various toxins such as fumonisins and T2-toxins. Among the Toxigens, Fusarium verticillioides (Gibberella moniliformis) is intensely toxigen. This fungus produces B1, B2, B3 fumonisins on some crops. The purpose of this study is the identification of an electrophoretic cytoplasmic protein pattern of Iranian Fusarium verticillioides.

Material and Methods: In this study 20 isolates of this species were analyzed. Using the Bradford method was measured protein range of each isolate and obtained its’ molecular weight by SDS-PAGE.

Results: The results indicated total 50 protein bands with molecular weight from 7 to 157 KD. Maximum protein bands were related to F4 and F10-c isolates with moderate toxigenicity and minimum protein bands to M2-a, K6 and A7-b isolates with Low, moderate and high toxigenicities.

Conclusion: The comparison of the electrophoretic cytoplasmic protein pattern of isolates with grouping based on toxigenicity did not show any correlation between their protein pattern and range of toxigenicity.

Keywords: protein pattern, Fusaium verticillioides, SDS-PAGE

Corresponding Author: Daie Ghazvini, R.

Email: roshanak1043@yahoo.com

Received: 20 Feb 2015
Revised: 16 Mar 2015
Accepted: 6 Apr 2015

This paper should be cited as: Daie Ghazvini R, Khosravi A, Ghiasian SA. [Identification of Electrophoretic Protein Pattern of Fusaium Verticillioides Isolates from Maize by SDS-PAGE]. mljgoums. 2015; 9(1):61-69 [Article in Persian]