This paper should be cited as: Khoramabadi, N. Mohabati Mobarez, A. Tabaraie, B. Behmanesh, M Atyabi, F. Aghababa, H.

rBCSP31 Antibody Response in Patients with Brucellosis: A Candidate for Brucella Vaccine

 

Khoramabadi, N. (PhD)1 Mohabati Mobarez, A. (PhD)2 Tabaraie, B. (PhD)3 Behmanesh, M. (PhD)4 Atyabi, F. (PhD)5 Aghababa, H. (MSc)6

 

 

1. Assistant Professor of Medical Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

2. Associate Professor of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

3. Assistant Professor of Microbiology, Pasteur Institute of Iran, Tehran, Iran

4. Associate Professor of Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran

5. Professor of Pharmacology, Nanotechnology Research Center, Tehran University of Medical Sciences, Tehran, Iran

6. MSc of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

 

Abstract

 

Background and Objective: One of the proteins shared in all strains of Brucella is 31 kDa surface protein (BCPS31) that could be an appropriate target for immunization and serological diagnosis.

Material and Methods: In the present study, BCSP31 produced as a recombinant protein in pET28a (+) expression system was utilized, using ELISA, to detect trace specific antibody (IgG) in brucellosis patients' serum that was confirmed by culture. We also evaluated cytokine response of peripheral blood lymphocytes to this protein in the cell culture.

Results: The results indicated a significant amount of surface protein antibodies (IgG) in the serum of patients with brucellosis. Evaluation of lymphocyte responses to rBCSP31 also showed a significant IL-12 and IFN-γ production in patients’ lymphocyte cultures.

Conclusion: These results suggest that BCSP31 can elicit specific humoral and cellular responses during host infection and it can be used in designing immunization and serologic diagnosis systems.

Keywords: Brucellosis, 31 kDa Cell Surface Protein, Brucella, Cytokine

 

 

Corresponding Author: Mohabati Mobarez, A.

 

Email: mmmobarez@modares.ac.ir

 

Received: 31 Aug 2013
Revised: 27 Sep 2013
Accepted: 30 Sep 2013