This paper should be cited as:Forouzandeh,S, Naghsh,N, Salimi, S,Jahantigh, D.
Cytotoxic Effect of Boswellia Serrata Hydroalcholic Extract on Human Cervical Carcinoma Epithelial Cell Line
Forouzandeh, S. (MSc)*1, Naghsh, N. (PhD)2, Salimi, S. (PhD)3, Jahantigh, D. (MSc)4
1. MSc of Animal Phsiology,
Islamic Azad University, Falavarjan Branch, Isfahan,
Iran
2. Assistant Professor of Animal
physiology, Islamic Azad University, Falavarjan
Branch, Isfahan, Iran
3. Associated Professor of Biochemistry,
Cellular and Molecular Research Center, Zahedan University of Medical
Sciences, Zahedan, Iran
4. MSc of Cellular and Molecular Biology,
Cellular and Molecular Research Center, Zahedan University of
Medical Sciences, Zahedan, Iran
Abstract
Background and Objective: Cervical cancer is the
second most common cancer in women.Boswellia serrata is a medicinal herb with anticancer, antibacterial,
antiulcer, antifungal properties.Since the antitumor
effect of this medicine has not been studied on cancer cell lines,
we aimed to investigate the antitumor effect of Boswellia
serrata on cervical cancer cell lines.
Material and
Methods: To
assess the anti-cancer effect of Boswellia serrata extract, HeLa cell lines
were cultured ,propagated and placed with different doses of Boswellia serrata (12.5,25, 50
and100 µg/ml) for 24,48and72 hours. After that, MTT test was used to determine the
cellular toxicity of the extract.
Results: The results of the MTT
test showed that this extract has dose-dependent and time-dependent anti
cancer effect on Hela in that the highest
effect was seen with 100 µg/ml of extract for 72 hrs. The half maximal inhibitory concentration (IC50) for 24
and 48 hrs were 12.5 and 50 µg/ml, respectively.In 72 hours, due to increase of incubation
period in all concentrations, the number of killed cells was more
than 50 percent.Consequently, IC50 was
not observed for this period of time.
Conclusion:Considering dose-dependent and time-dependent anti
cancer effect, Boswellia serrata
extract can inhibit the growth of Hela cells.
Keywords:Hela Cell; MTT Test; Boswellia Serrata Extract;
Cervical Cancer
Corresponding
Author: Forouzandeh, S.
Email:
forouzan.forouzandeh @yahoo.com
Received:
20 Jan 2013 Revised: 8 Feb 2013 Accepted: 9 Feb 2013