Volume 11, Issue 1 (Jan-Feb- 2017 2017)                   mljgoums 2017, 11(1): 23-27 | Back to browse issues page

DOI: 10.18869/acadpub.mlj.11.1.23

XML Print

1- Department of Botany , Mazandarani.M@gorganiau.ac.ir
2- Department of Biology, Islamic Azad University, Gorgan Branch, Gorgan, Iran
Abstract:   (14300 Views)
          Background and Objectives: Many aromatic plants from the genus Satureja have been used in traditional medicine in north of Iran. This study aimed to determine the ecological requirements for the growth of Satureja mutica Fisch. & C.A. Mey, and evaluate antioxidant and antibacterial activity of ethanolic extract of S. mutica collected from North Khorasan Province, Iran.
         Methods: Aerial parts of S. mutica were collected in blooming stage. Ecological requirements and the traditional uses of the plant were recorded. Ethanol extract of the plant was prepared by maceration. Antioxidant capacity of the extract was measured by three methods of total antioxidant capacity, reducing power and 2,2-Diphenyl-1-picrylhydrazyl, and then compared with standard antioxidants (butylated hydroxyanisole and butylated hydroxytoluene). Antibacterial activity of the extract was studied against nine Gram-positive and Gram-negative bacteria by agar dilution method and determining the minimum inhibitory concentrations (MICs).
         Results: S. mutica is the most common wild aromatic annual herb in north slob and sunny areas around mountains of Bojnord (1020-1300 m). The ecological features of this region are as follows: annual rainfall 308 mm, average temperature 11.5 oC, semi dry cold climate in the sandy clay loam soil, Ec=0.7 desizimence, and pH= 7.30. Ethnopharmacological data showed that this plant has been widely used by rural people as an anti-infective, antispasm and sedative agent that could treat rheumatic pain, migraine, toothache and diarrhea. The ethanol extract of S.mutica had relatively high antioxidant activity with IC50 value of 11.2 mg/ml. The extract also had high antibacterial activity against Staphylococcus aureus, Staphylococcus epidermidis, Bacillus cereus and Enterococcus faecalis, with inhibition zone diameters ranging between 15.1±0.5 and 27.7±0.8 mm and MIC values of 60, 68, 53 and 83 mg/ml, respectively.
         Conclusion: It can be concluded that the extract of S. mutica has favorable antibacterial and antioxidant activity, which could be used as natural anti-microbial agent for treatment of some infection diseases.
          Keywords: Antioxidant, Antimicrobial, Bojnord, Ecological Requirements, North Khorasan, BHT, BHA.
Full-Text [PDF 450 kb]   (953 Downloads)    
Type of Study: Original Paper |
Received: 2017/01/28 | Accepted: 2017/01/28 | Published: 2017/01/28 | ePublished: 2017/01/28

1. Mazandarani M, Makari S, Bajian GhR, Zarghami Moghaddam P, Abrodi M. Evaluation of phytochemical and antioxidant activity in different parts of Heracleum gorganicum Rech. in Golestan province of Iran. Iranin Jplant physiol. 2011;2(2):381-386.
2. Tepe B, Daferera D, Sokmen A, Sokmen M, Polissiou M. Antimicrobial and antioxidant activities of the essential oil and various extracts of Salvia tomentosa Miller. (Lamiaceae). Food Chem. 2005;90(3):333-40. [DOI:10.1016/j.foodchem.2003.09.013]
3. Sadeghi I, Yousefzadi M, Behmanesh M, Sharifi M.In vitro cytotoxic and antimicrobial activity of essential oil from Satureja Intermedia. Iranian Red Crescent Medical Journal. 2013; 15(1): 70-4. [DOI:10.5812/ircmj.4989]
4. Rechinger KH, Satureja. In: Cavaleiro C, Salgueiro LR, Antunes T, editor(s). 1980. Flora Iranica. Graz, Austria: Sevinate.
5. MozaffarianVA. Dictionary of Iranian plant names. Tehran, Iran: Farhang Moaser. 1996.
6. Tzakou O, Skaltsa H.Composition and antibacterial activity of the essential oil of Satureja parnassica subsp parnassica. Planta Med. 2003;69(3):282-4. [DOI:10.1055/s-2003-38487]
7. Adiguzel A, Ozer H, KiliC H, CetiN B.Screening of antimicrobial activity of essential oil and methanol extract of Satureja hortensis on foodborne bacteria and fungi. . Czech J Food Sci. 2007;25(2):81-89.
8. Ghannadi A. Composition of the essential oil of Satureja hortensis L. seeds from Iran. J Essent Oil Res. 2002;14(1):35-6. [DOI:10.1080/10412905.2002.9699754]
9. Kurkcuoglu M, Tumen G, Baser KHC. Essential oil constituents of Satureja boissieri from Turkey. Chem Nat Comp.2001; 37:329-31.DOI: 10.1023/A:1013714316862. [DOI:10.1023/A:1013714316862]
10. Hadian J, Ebrahimi SN, Salehi P.Variability of morphological and phytochemical characteristics among Satureja hortensis L. accessions of Iran. Ind Crop Prod. 2010;32(1):62-9. [DOI:10.1016/j.indcrop.2010.03.006]
11. Sefidkon F, Jamzad Z.Chemical composition of the essential oil of three Iranian Satureja species (S. mutica, S. macrantha and S. intermedia). Food Chem. 2005;91(1):1-4. [DOI:10.1016/j.foodchem.2004.01.027]
12. Ciani M, Menghini L, Mariani F, Pagiotti R, Menghini A, Fatichenti F. Antimicrobial properties of essential oil of Satureja montana L. on pathogenic and spoilage yeasts. Biotechnology Letters.2002; 22(12): 1007. DOI: 10.1023/A:1005649506369. [DOI:10.1023/A:1005649506369]
13. Eftekhar F, Raei F, Yousefzadi M, Ebrahimi SN, Hadian J.Antibacterial activity and essential oil composition of Satureja spicigera from Iran. Z Naturforsch C. 2009;64(1-2):20-4. [DOI:10.1515/znc-2009-1-204]
14. Ćavar S, Maksimović M, Šolić ME, Jerković-Mujkić A, Bešta R. Chemical composition and antioxidant and antimicrobial activity of two Satureja essential oils. Food Chem. 2008;111(3):648-53. [DOI:10.1016/j.foodchem.2008.04.033]
15. Pourmorad F, Hosseinimehr SJ, Shahabimajd N. Antioxidant activity, phenol and flavonoid contents of some selected Iranian medicinal plants. Afr Jof Biotechnology. 2006; 5(11):1142-1145.
16. Arabshahi-DeloueeS, Uroo A.Antioxidant propperties of various solvent extracts of mulberry (Morus indical L.) leaves. Food chemistry. 2007; 102(4):1233-1240.doi:10.1016/j.foodchem.2006.07.013. [DOI:10.1016/j.foodchem.2006.07.013]
17. Ketema T, Kaleab A, Fathy EF, Abdel Nasser S, BucarB. Composition of the essential oils of Satureja abyssinica ssp. abyssinica and Satureja paradoxa: Their antimicrobial and radical scavenging activities. Journal of Essential Oil Research.2007; 19 (3): 295-300. [DOI:10.1080/10412905.2007.9699285]
18. Sonboli A, Fakhari A, Kanani MR, Yousefzadi M. Antimicrobial activity, essential oil composition and micromorphology of trichomes of Satureja laxiflora C. Koch from Iran. Z Naturforsch C.2004;59(11):777-81. [DOI:10.1515/znc-2004-11-1202]
19. Vagionas K, Graikou K, Ngassapa O, Runyoro D, Chinou I.Composition and antimicrobial activity of the essential oils of three Satureja species growing in Tanzania. Food Chem;2007. 103(2):319-24. [DOI:10.1016/j.foodchem.2006.07.051]
20. Oke F, Aslim B, Ozturk S, Altundag S. Essential oil composition, antimicrobial and antioxidant activities of Satureja cuneifolia Ten. Food Chem. 2009;112(4):874-9. [DOI:10.1016/j.foodchem.2008.06.061]
21. Sarkhail P, Rahmani P. Hypoglycemic Property of Satureja khuzestanica in Human Still in Doubt. international Journal of Pharmacology.2011; 7(7): 745-6.DOI: 10.3923/ijp.2011.745.746. [DOI:10.3923/ijp.2011.745.746]
22. Askari F, Sefidkon F, Sadeghzadeh L, Olia P.Essential oil composition and antibacterial activity of three species of Satureja against Salmonella typhimurium. Iranian Biology Journal.2008; 22(2): 242-258.
23. Serrano C, Matos O, Teixeira B, Ramos C, Neng N, NogueiraJ,et al. Antioxidant and antimicrobial activity of Saturejamontana L. extracts. Journal of the Science of Food and Agriculture. 2011;91(9): 1554-1560. [DOI:10.1002/jsfa.4347]
24. Khadivi-khubA, Salehi Arjmand H, Hadian J.Morphological and phytochemical variation of Satureja bachtiarica populations from Iran.Industrial Crops and Products. 2014; 54:257-265. [DOI:10.1016/j.indcrop.2014.01.039]
25. Gohari AR, Hadjiakhoondi A, ShafieeA, Ebrahimi ES, Mozaffarian V. Chemical Composition of the Essential Oils of Satureja atropatana and Satureja mutica Growing Wild in Iran. Journal of Essential Oil Research.2005; 17(1): 17-18. [DOI:10.1080/10412905.2005.9698817]
26. Baser KHC, Özek T, Kirimer N, Tümen G. Comparative Study of the Essential Oils of Wild and Cultivated Satureja hortensis L. Journal of Essential Oil Research.2004; 16(5): 422-424. [DOI:10.1080/10412905.2004.9698761]
27. Tabatabaei-Raisi A, Delazar A, Khaligi A, Kaviani B, Hashemabadi D. Variability of Essentials Oils of Various Parts of Satureja sahendica Bornm. and Their Antioxidant Activity. international Journal of Botany.2008; 4(2): 245-248. DOI: 10.3923/ijb.2008.245.248. [DOI:10.3923/ijb.2008.245.248]
28. Safarnavadeh T, Rastegarpanah M. Antioxidants and infertility treatment, the role of Satureja khuzestanica: A mini-systematic review. Iranian Journal of Reproductive Medicine; . 2011; 9(2): 61-70.
29. Skočibuašić M, Nada B. Chemical Composition and Antimicrobial Variability of Satureja montana L. Essential Oils Produced During Ontogenesis. Journal of Essential Oil Research.2004; 16(4): 387. [DOI:10.1080/10412905.2004.9698751]