Volume 10, Issue 6 (Nov-Dec-2016 2016)                   mljgoums 2016, 10(6): 49-53 | Back to browse issues page

‎ 10.18869/acadpub.mlj.10.6.49

XML Print

Evaluation of Antibacterial Effects of Aqueous and Alcoholic Extracts of Nasturtium Officinale on Some Pathogenic
Saeid Farshbaf Derhami1 , Mehdi Ghiami Rad1 , Razzagh Mahmoudi 2
1- Department of Microbiology, Faculty of Basic science Biology
2- Department of Food Hygiene and Aquatics, Faculty of Veterinary Medicine
Abstract:   (14705 Views)

ABSTRACT

         Background and Objective: Although antibiotics are commonly used for treatment of infectious diseases, these treatments are often associated with several problems such as unwanted side effects and resistance to antibiotics. The aim of this study was to evaluate the antibacterial effect of aqueous and alcoholic extracts of Nasturtium officinale on Escherichia coli, Salmonella typhimurium, Staphylococcus aureus and Listeria monocytogenes.

          Methods: All experiments were performed using the well diffusion method. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the aqueous and alcoholic extracts of N. officinale against the pathogenic bacteria studied were determined by microdilution method.

         Results: In the well diffusion method, S. aureus and L. monocytogenes were the most sensitive bacteria with MIC of 8 μg/ml, while E. coli and S. typhimurium were the most resistant bacteria to the aqueous and alcoholic extracts. In addition, the inhibitory activity of the alcoholic and aqueous extracts of N. officinale was higher against gram-positive bacteria compared to gram-negatives. The lowest MIC (6.25 μg/ml) and MBC (12.5 μg/ml) of the plant extract were against S. aureus.

          Conclusion: The aqueous and alcoholic extracts of N. officinale affect the growth of gram-positive bacteria (S. aureus and L. monocytogenes) but not the gram-negatives (S. typhimurium and E. coli). These extract could be used for treatment of infections.

        Keywords: Nasturtium Officinale, Aqueous Extract, Alcoholic Extract, Antibacterial Effect.

Keywords: Nasturtium Officinale, Aqueous Extract, Alcoholic Extract, Antibacterial Effect.
Full-Text [PDF 595 kb]   (1643 Downloads)    
Research Article: Original Paper |
Received: 2016/01/25 | Accepted: 2016/01/25 | Published: 2016/09/25 | ePublished: 2016/09/25
References
1. Ghaderi S, Falahati A, Sarailoo MH. Investigation of the components and antibacterial effects of three plant's essential oil Coriandrum sativum, Achillea millefolium, Anethum graveolens in vitro. Journal of Shahrekord University of Medical Sciences. 2012; 14(5): 74-82. [Persian]
2. Sefidkon F, Torabi B, Naderi M. Comparison of anticancer effects of nanocapsules of Nasturtium officinalis (L.) R. Br. extract with methanolic extract and its fractions. Iranian Journal of Medicinal and Aromatic Plants. 2013; 29(1): 35-50[Persian].
3. Moradian H, Bazargani A, Rafiee A, Nazarialam A. In vitro comparison of antimicrobial activity of aqueous decoction of Coriandrum sativum, and Dentol Drop with chlorhexidine on Streptococcus mutans. Iran J Microbiol. 2013; 5(3): 239-43.
4. Shahroki N, Khaksari M, Shabani M. The Effect of seeding Nasturtium officinale water Extract on plasma lipids and Glucose level diabetic rat. Journal of Rafsanjan University of Medical Sciences, 2007. 6(4): 245-54[Persian].
5. Jafari AA, Shohrati M, Mahmoudi R, Haj Hoseini R, Nosratpour S, Pajohi-Alamoti M, Mohamad Latifi A. Chemical composition and biological activities of Scrophularia striata extracts. Minerva Biotecnologica. 2014; 26 (3): 183-189.
6. Shoae Hassani AR, Ordouzadeh N, Ghaemi A, Nazari R, Hamdi K, Hekmatpou D. Comparing Black and Green tea (Camellia sinensis L) extractseffects on the growth inhibition and biofilm formation of Enterobacteriacea. Journal of Arak University of Medical Sciences. 2008; 11(2): 64-73[Persian].
7. Gulluce M, Sahin F, Sokman M, Ozer H, Daferera D, Sokman A. Antimicrobial and antioxidant properties of the essential oils and methanol extract from Mentha longifolia L. longifolia. Food Chemistry. 2007; 103(4): 1449-1456. [DOI:10.1016/j.foodchem.2006.10.061]
8. Eloff, JN. It is possible to use herbarium specimens to screen for antibacterial components in some plant. J Ethno pharmacol. 1999. 67(3): 355-60. [DOI:10.1016/S0378-8741(99)00053-7]
9. Shairfa AA, Neoh YL, Iswadi MI, Khairul O, Abdul Halim M, Jamaludin M, et al. Effects of Methanol, Ethanol and Aqueous Extract of Plantago major on Gram Positive Bacteria. Gram Negative Bacteria and Yeast. 2008; 8: 42-44.
10. Jalali M. Antimicrobial effects of ethanol extracts of some medicinal plant against Listeria monosytogenes. Journal of Shahrekord University of Medical Sciences. 2008; 8(3): 23-25. [Persian]
Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.