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Arefi M, Abdollahi A, Khosravi A, Moradi A, Aghaee-Bakhtiari S H, Javid N, et al . Expression Analysis of Circulating miR-21 in Iranian Patients with Hereditary Colorectal Cancer. mljgoums 2021; 15 (2) :1-4
URL: http://mlj.goums.ac.ir/article-1-1262-en.html
1- Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Golestan University of Medical Sciences, Gorgan, Iran
2- Department of General Surgery, School of Medicine, Surgical Oncology Research Center, Ghaem Hospital, Mashhad University of Medical Sciences, Mashhad, Iran
3- Department of Molecular Medicine, School of Advanced Technologies in Medicine, Golestan University of Medical Sciences, Gorgan, Iran
4- Department of Microbiology, School of Medicine, Research Center of Gastroenterology and Hepatology, Golestan University of Medical Sciences, Gorgan, Iran
5- Department of Medical Biotechnology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
6- Department of Pharmaceutical Biotechnology, School of Pharmacy, Guilan University of Medical Sciences, Rasht, Iran
7- Medical Cellular and Molecular Research Center, Golestan University of Medical Sciences, Gorgan, Iran , kiabiotpro@yahoo.com
Abstract:   (2500 Views)
Background and objectives: Colorectal cancer (CRC) is one of the leading causes of cancer-related mortality in the world. MicroRNAs (miRNAs) have potential as diagnostic biomarkers for various diseases including cancer. This study was undertaken to investigate expression of miR-21 before and after surgery in patients with hereditary CRC.
Methods: After collecting blood samples from 39 patients and 39 healthy controls, total RNA was extracted by the TRIzol method. Following cDNA synthesis, expression of miR-21 in serum of subjects was evaluated using real-time PCR, along with two reference genes, let-7d and let-7g. The real-time expression results and Ct values were collected and analyzed based on the 2-∆∆ct method.
Results: In spite of tumor removal, serum miR-21 expression levels was significantly higher in hereditary CRC patients compared with controls (P=0.022).
Conclusion: Our results confirmed that samples from hereditary cases of CRC must not be included in experiments on the diagnostic potential of miRNAs.
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Research Article: Original Paper | Subject: Molecular Medicine
Received: 2019/11/5 | Accepted: 2019/12/14 | Published: 2021/02/28 | ePublished: 2021/02/28

References
1. Marley AR, Nan H. Epidemiology of colorectal cancer. International journal of molecular epidemiology and genetics. 2016; 7: 105-114. [PubMed] [Google Scholar]
2. Kolligs FT. Diagnostics and epidemiology of colorectal cancer. Visceral medicine. 2016; 32: 158-164. [DOI:10.1159/000446488] [PubMed] [Google Scholar]
3. Aran V, Victorino AP, Thuler LC , Ferreira CG . Colorectal cancer: epidemiology, disease mechanisms and interventions to reduce onset and mortality. Clinical Colorectal Cancer. 2016; 15: 195-203. [DOI:10.1016/j.clcc.2016.02.008] [PubMed] [Google Scholar]
4. Chen W, Zheng R, Baa de PD, Zhang S, Zeng H, Bray F, et al. Cancer statistics in China, 2015. CA: A Cancer Journal for Clinicians. 2016; 66: 115-132. [DOI:10.3322/caac.21338] [PubMed] [Google Scholar]
5. Hamada T, Nishihara R, Ogino S. Post-colonoscopy colorectal cancer: the key role of molecular pathological epidemiology. Translational gastroenterology and hepatology. 2017; 2: 9. [DOI:10.21037/tgh.2017.01.05] [PubMed] [Google Scholar]
6. Reddy KB. MicroRNA (miRNA) in cancer. Cancer cell international. 2015; 15: 38. [DOI:10.1186/s12935-015-0185-1] [PubMed] [Google Scholar]
7. Sun Y, Liu Y, Cogdell D, Calin GA, Sun B, Kopetz S, et al. Examining plasma microRNA markers for colorectal cancer at different stages. Oncotarget. 2016; 7: 11434-11449. [DOI:10.18632/oncotarget.7196] [PubMed] [Google Scholar]
8. Wang S, Xiang J, Li Z, Lu S, Hu J, Gao X, et al. A plasma microRNA panel for early detection of colorectal cancer. International journal of cancer. 2015; 136(1): 152-161. [DOI:10.1002/ijc.28136] [PubMed] [Google Scholar]
9. Fang Z, Tang J, Bai Y, Lin H, You H, Jin H, et al. Plasma levels of microRNA-24, microRNA-320a, and microRNA-423-5p are potential biomarkers for colorectal carcinoma. Journal of experimental and clinical cancer research. 2015; 34: 86. [DOI:10.1186/s13046-015-0198-6] [PubMed] [Google Scholar]
10. Asaga S, Kuo C, Nguyen T, Terpenning M, Giuliano AE, Hoon DS. Direct serum assay for microRNA-21 concentrations in early and advanced breast cancer. Clinical chemistry. 2011; 57: 84-91. [DOI:10.1373/clinchem.2010.151845] [PubMed] [Google Scholar]
11. Kanaan Z, Rai SN, Eichenberger MR, Roberts H, Keskey B, Pan J, et al. Plasma MiR-21: a potential diagnostic marker of colorectal cancer. Annals of Surgery. 2012; 256: 544-51. [DOI:10.1097/SLA.0b013e318265bd6f] [PubMed] [Google Scholar]
12. Mitchell PS, Parkin RK, Kroh EM, Fritz BR, Wyman SK, Pogosova-Agadjanyan EL, et al. Circulating microRNAs as stable bloodbased markers for cancer detection. Proceedings of the national academy of sciences. U.S.A. 2008; 105(30): 10513-8. [DOI:10.1073/pnas.0804549105] [PubMed] [Google Scholar]
13. Chen X, Ba Y, Ma L, Cai X, Yin Y, Wang K, et al, et al. Characterization of microRNAs in serum: a novel class of biomarkers for diagnosis of cancer and other diseases. Cell Research. 2008; 18(10): 997-1006. [DOI:10.1038/cr.2008.282] [PubMed] [Google Scholar]
14. Gilad S, Meiri E, Yogev Y, Benjamin S, Lebanony D, Yerushalmi N, et al. Serum microRNAs are promising novel biomarkers. PLoS ONE. 2008; 3: e3148. [DOI:10.1371/journal.pone.0003148] [PubMed] [Google Scholar]
15. Toiyama Y, Takahashi M, Hur K, Nagasaka T, Tanaka K, Inoue Y, et al. Serum miR-21 as a diagnostic and prognostic biomarker in colorectal cancer. Journal of the national cancer institute. 2013; 105(12): 849-59. [DOI:10.1093/jnci/djt101] [PubMed] [Google Scholar]

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