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mljgoums 2020, 14(1): 15-19 |
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Ebrahimnezhad H, Barzegar L, Esmaeili D. Antibacterial Effects of Compound Bifilact on E.coli and Campylobacter jejuni. mljgoums 2020; 14 (1) :15-19
URL: http://mlj.goums.ac.ir/article-1-1206-en.html
URL: http://mlj.goums.ac.ir/article-1-1206-en.html
1- Student of pharmacy, Department of Pharmaceutics, Baqiyatallah University of Medical Sciences,Tehran,Iran
2- Department of Microbiology and Applied Microbiology Research Center, Systems Biology and Poisonings Institute, BaqiyatallahUniversity of Medical Sciences, Tehran, Iran
3- Applied VirologyResearch Center, BaqiyatallahUniversity of Medical Sciences, Tehran, Iran, and Department of Microbiology and Applied Microbiology Research Center, Systems Biology and Poisonings Institute, BaqiyatallahUniversity ofMedical Sciences, Tehran, Iran , esm114@gmail.com
2- Department of Microbiology and Applied Microbiology Research Center, Systems Biology and Poisonings Institute, BaqiyatallahUniversity of Medical Sciences, Tehran, Iran
3- Applied VirologyResearch Center, BaqiyatallahUniversity of Medical Sciences, Tehran, Iran, and Department of Microbiology and Applied Microbiology Research Center, Systems Biology and Poisonings Institute, BaqiyatallahUniversity ofMedical Sciences, Tehran, Iran , esm114@gmail.com
Abstract: (4369 Views)
ABSTRACT
Background and Objectives: Probiotics are live microorganisms that function through various mechanisms and affect the alteration of the commensal microbiota against pathogens. Nowadays, given the problems associated with antibiotics use, probiotic strains offer a novel and appropriate alternative for the treatment of diseases such as diarrhea. The aim of this study was to investigate the antibacterial synergism of Lactobacillus spp., Bifidobacterium spp. and Escherichia coli strain Nissle 1917 (ECN) on the clinical sample of diarrheagenic E.coli and Campylobacter jejuni.
Methods: A paper disk-diffusion technique was used to evaluate the antibacterial activity. Sterile 6 mm paper disks were saturated with probiotic suspensions made by settling probiotic medications into distilled water. Three kinds of disk were prepared. One disk was prepared for Lactobacillus spp. and Bifidobacterium spp., another for ECN, and the third was made by combined probiotics. Clinical samples of diarrheagenic E.coli and Campylobacter jejuni were cultivated on Muller Hinton agars, and disks were placed on the inoculated Muller Hinton agars. All plates were incubated under microaerophilic and appropriate conditions.
Results: The zone of inhibition (ZOI) of the bacterial growth was measured. All pathogenic microorganisms showed sensitivity to the probiotic disks. The combined disks had better effects against pathogens compared with single disks.
Conclusion: A considerable synergistic effect was observed in the results of combined probiotics; therefore, combined strains can be more efficient against intestinal pathogens in comparison with single probiotics.
Keywords: Probiotic, Lactobacillus, Bifidobacterium, Escherichia coli Nissle, Diarrhea, Campylobacter jejun.i.
Background and Objectives: Probiotics are live microorganisms that function through various mechanisms and affect the alteration of the commensal microbiota against pathogens. Nowadays, given the problems associated with antibiotics use, probiotic strains offer a novel and appropriate alternative for the treatment of diseases such as diarrhea. The aim of this study was to investigate the antibacterial synergism of Lactobacillus spp., Bifidobacterium spp. and Escherichia coli strain Nissle 1917 (ECN) on the clinical sample of diarrheagenic E.coli and Campylobacter jejuni.
Methods: A paper disk-diffusion technique was used to evaluate the antibacterial activity. Sterile 6 mm paper disks were saturated with probiotic suspensions made by settling probiotic medications into distilled water. Three kinds of disk were prepared. One disk was prepared for Lactobacillus spp. and Bifidobacterium spp., another for ECN, and the third was made by combined probiotics. Clinical samples of diarrheagenic E.coli and Campylobacter jejuni were cultivated on Muller Hinton agars, and disks were placed on the inoculated Muller Hinton agars. All plates were incubated under microaerophilic and appropriate conditions.
Results: The zone of inhibition (ZOI) of the bacterial growth was measured. All pathogenic microorganisms showed sensitivity to the probiotic disks. The combined disks had better effects against pathogens compared with single disks.
Conclusion: A considerable synergistic effect was observed in the results of combined probiotics; therefore, combined strains can be more efficient against intestinal pathogens in comparison with single probiotics.
Keywords: Probiotic, Lactobacillus, Bifidobacterium, Escherichia coli Nissle, Diarrhea, Campylobacter jejun.i.
Keywords: Probiotic, Lactobacillus, Bifidobacterium, Escherichia coli Nissle 1917, diarrhea, Campylobacter jejuni
Research Article: Original Paper |
Subject:
Microbiology
Received: 2019/03/22 | Accepted: 2019/07/16 | Published: 2019/12/30 | ePublished: 2019/12/30
Received: 2019/03/22 | Accepted: 2019/07/16 | Published: 2019/12/30 | ePublished: 2019/12/30
References
1. Henker J, Laass M, Blokhin BM, Bolbot YK, Maydannik VG, Elze M, et al. The probiotic Escherichia coli strain Nissle 1917 (EcN) stops acute diarrhea in infants and toddlers. Eur J Pediatr. 2007; 166(4): 311-8. [DOI:10.1007/s00431-007-0419-x]
2. Sanders ME, Guarner F, Guerrant R, Holt PR, Quigley EM, Sartor RB, et al. An update on the use and investigation of probiotics in health and disease. Gut. 2013; 62(5): 787-796. [DOI:10.1136/gutjnl-2012-302504]
3. Maltby R, Leatham-Jensen MP, Gibson T, Cohen PS, Conway T, et al. Nutritional Basis for Colonization Resistance by Human Commensal Escherichia coli Strains HS and Nissle 1917 against E. coli O157: H7 in the Mouse Intestine. PLOS ONE. 2013; 8(1): p. e53957. [DOI:10.1371/journal.pone.0053957]
4. Fayol-Messaoudi D1, Berger CN, Coconnier-Polter MH, Liévin-Le Moal V, Servin AL. pH-, Lactic Acid-, and Non-Lactic Acid-Dependent Activities of Probiotic Lactobacilli against Salmonella enterica Serovar Typhimurium. Applied and Environmental Microbiology. 2005; 71(10): 6008-6013. [DOI:10.1128/AEM.71.10.6008-6013.2005]
5. Corr SC, Li Y, Riedel CU, O'Toole PW, Hill C, Gahan CG. Bacteriocin production as a mechanism for the antiinfective activity of Lactobacillus salivarius UCC118. Proceedings of the National Academy of Sciences. 2007; 104(18): 7617-7621. [DOI:10.1073/pnas.0700440104]
6. Jones SA, Gibson T, Maltby RC, Chowdhury FZ, Stewart V, Cohen PS, et al. Anaerobic respiration of Escherichia coli in the mouse intestine. Infect Immun. 2011; 79(10): 4218-26. doi: 10.1128/IAI.05395-11. [DOI:10.1128/IAI.05395-11]
7. Boudeau J, Glasser AL, Julien S, Colombel JF, Darfeuille-Michaud A. Inhibitory effect of probiotic Escherichia coli strain Nissle 1917 on adhesion to and invasion of intestinal epithelial cells by adherent-invasive E. coli strains isolated from patients with Crohn's disease. Aliment Pharmacol Ther. 2003; 18(1): 45-56. [DOI:10.1046/j.1365-2036.2003.01638.x]
8. Corthésy B, Gaskins HR, Mercenier A. Gaskins, and A. Mercenier, Cross-Talk between Probiotic Bacteria and the Host Immune System. J Nutr. 2007; 137(3 Suppl 2): 781S-90S. doi: 10.1093/jn/137.3.781S. [DOI:10.1093/jn/137.3.781S]
9. Varankovich NV, Nickerson MT, Korber DR. Probiotic-based strategies for therapeutic and prophylactic use against multiple gastrointestinal diseases. Front Microbiol. 2015; 6: 685. doi: 10.3389/fmicb.2015.00685. [DOI:10.3389/fmicb.2015.00685]
10. Kaakoush NO, Castaño-Rodríguez N, Mitchell HM, Man SM. Global Epidemiology of Campylobacter Infection. Clin Microbiol Rev. 2015; 28(3): 687-720. [DOI:10.1128/CMR.00006-15]
11. Weintraub A. Enteroaggregative Escherichia coli: epidemiology, virulence and detection. J Med Microbiol. 2007; 56(Pt 1): 4-8. [DOI:10.1099/jmm.0.46930-0]
12. Iijima Y, Oundo JO, Hibino T, Saidi SM, Hinenoya A, Osawa K, et al. High Prevalence of Diarrheagenic Escherichia coli among Children with Diarrhea in Kenya. Jpn J Infect Dis. 2017; 70(1): 80-83. doi: 10.7883/yoken.JJID.2016.064. [DOI:10.7883/yoken.JJID.2016.064]
13. Benmessaoud, R.; Jroundi, I.; Nezha, M.; Moraleda, C.; Tligui, H.; Seffar, M.; Alvarez-Martínez, M.J.; Pons, M.J.; Chaacho, S.; Hayes, E.B.; et al. Aetiology, epidemiology and clinical characteristics of acute moderate-to-severe diarrhoea in children under 5 years of age hospitalized in a referral paediatric hospital in Rabat, Morocco. J. Med. Microbiol. 2015, 64, 84-92 [DOI:10.1099/jmm.0.079830-0]
14. Amour C, Gratz J, Mduma E, Svensen E, Rogawski ET, McGrath M, et al. Epidemiology and Impact of Campylobacter Infection in Children in 8 Low-Resource Settings: Results From the MAL-ED Study. Clinical Infectious Diseases. 2016; 63(9): 1171-1179. [DOI:10.1093/cid/ciw542]
15. Silva MT, Simas SM, Batista TG, Cardarelli P, Tomassini TC, et al. Studies on antimicrobial activity, in vitro, of Physalis angulata L. (Solanaceae) fraction and physalin B bringing out the importance of assay determination. Mem Inst Oswaldo Cruz. 2005; 100(7): 779-82. [DOI:10.1590/S0074-02762005000700018]
16. Nijs A, Cartuyvels R, Mewis A, Peeters V, Rummens JL, Magerman K. Comparison and Evaluation of Osiris and Sirscan 2000 Antimicrobial Susceptibility Systems in the Clinical Microbiology Laboratory. J Clin Microbiol. 2003; 41(8): 3627-30. [DOI:10.1128/JCM.41.8.3627-3630.2003]
17. Alakomi HL, Skyttä E, Saarela M, Mattila-Sandholm T, Latva-Kala K, Helander IM. Lactic Acid Permeabilizes Gram-Negative Bacteria by Disrupting the Outer Membrane. Appl Environ Microbiol. 2000; 66(5): 2001-5. [DOI:10.1128/AEM.66.5.2001-2005.2000]
18. Bratz K, Gölz G, Janczyk P, Nöckler K, Alter T. Analysis of in vitro and in vivo effects of probiotics against Campylobacter spp. Berl Munch Tierarztl Wochenschr. 2015; 128(3-4): 155-62.
19. Park SF. The physiology of Campylobacter species and its relevance to their role as foodborne pathogens. Int J Food Microbiol. 2002; 74(3): 177-88. [DOI:10.1016/S0168-1605(01)00678-X]
20. Lodinová-Zádniková R, Sonnenborn U. Effect of Preventive Administration of a Nonpathogenic Escherichia coli Strain on the Colonization of the Intestine with Microbial Pathogens in Newborn Infants. Biol Neonate. 1997; 71(4): 224-32. [DOI:10.1159/000244421]
21. Timmerman HM, Niers LE, Ridwan BU, Koning CJ, Mulder L, Akkermans LM, et al., Design of a multispecies probiotic mixture to prevent infectious complications in critically ill patients. Clinical Nutrition. 2007; 26(4): 450-459. [DOI:10.1016/j.clnu.2007.04.008]
22. Field D, Baghou I, Rea MC, Gardiner GE, Ross RP, Hill C. Nisin in Combination with Cinnamaldehyde and EDTA to Control Growth of Escherichia coli Strains of Swine Origin. Antibiotics (Basel). 2017; 6(4). pii: E35. doi: 10.3390/antibiotics6040035. [DOI:10.3390/antibiotics6040035]
23. Chi, H. and H. Holo (2017). "Synergistic Antimicrobial Activity Between the Broad Spectrum Bacteriocin Garvicin KS and Nisin, Farnesol and Polymyxin B Against Gram-Positive and Gram-Negative Bacteria." Current microbiology: 2017. 1-6. [DOI:10.1007/s00284-017-1375-y]
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