fa معرفی یک نسخه ساده از روش Van Erth در مطالعه SNP typing باسیلوس آنتراسیس سازگار با ماشین های ترموسایکلر معمولی Abstract SNP typing is now a well-established genotyping system in Bacillus anthracis studies. In the original standard method of Van Erth, SNPs at 13 loci of the B. anthracis genome were analyzed. In order to simplify and make appropriate this expensive method to low-budget laboratory settings, 13 primer pairs targeting the 13 corresponding SNPs were designed. Besides, a universal PCR protocol was developed to enable simultaneous amplification of all loci by conventional PCR machines. The efficiency of this approach was approved by applying on nine isolates of B. anthracis. We recommend using this modified procedure as an efficient alternative to Van Erth method until developing newer and affordable techniques. Keywords: Bacillus Anthracis, Genotyping, SNPs, PCR Bacillus Anthracis, Genotyping, SNPs, PCR 97 103 http://mlj.goums.ac.ir/browse.php?a_code=A-10-1-296&slc_lang=fa&sid=1 Najafi Olya, Z. (BSc) زهرا نجفی علیا 10031947532846008383 10031947532846008383 No Department of Veterinary Aerobic Bacteria, Razi Institute, Karaj,Iran مؤسسه تحقیقات واکسن و سرم سازی رازی Tadayon, K. (PhD) کیوان تدین k.tadayon@rvsri.ac.ir 10031947532846008384 10031947532846008384 Yes Department of Veterinary Aerobic Bacteria, Razi Institute, Karaj, Iran مؤسسه تحقیقات واکسن و سرم سازی رازی، کرج Ghaderi, R. (BSc) رایناک قادری 10031947532846008385 10031947532846008385 No Razi Institute, Karaj 3197619751, Iran مؤسسه تحقیقات واکسن و سرم سازی رازی، کرج